Differentiation of smoked Salmo salar, Oncorhynchus mykiss and Brama raii using the nuclear marker 5S rDNA

Summary PCR amplification of 5S rDNA was applied to differentiate smoked samples of Atlantic salmon ( Salmo salar ), rainbow trout ( Oncorhynchus mykiss ) and bream ( Brama raii ). Contiguous divergent primers for the conserved coding region of the 5S rRNA gene allowed species‐specific amplification...

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Bibliographic Details
Published in:International Journal of Food Science & Technology
Main Authors: Carrera, Esther, García, Teresa, Céspedes, Ana, González, Isabel, Fernández, Alicia, Asensio, Luis M., Hernández, Pablo E., Martín, Rosario
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2000
Subjects:
Brama
Atlantic salmon
Salmo salar
Online Access:http://dx.doi.org/10.1046/j.1365-2621.2000.00404.x
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Summary:Summary PCR amplification of 5S rDNA was applied to differentiate smoked samples of Atlantic salmon ( Salmo salar ), rainbow trout ( Oncorhynchus mykiss ) and bream ( Brama raii ). Contiguous divergent primers for the conserved coding region of the 5S rRNA gene allowed species‐specific amplification of tandemly arranged units of the 5S rDNA in these three related species. Thus, the 5S rDNA genetic marker may become very useful for inspection programmes intended to assess the species identity of smoked products.

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