Supercritical Fluids Are Superior Media for Catalysis by Cross‐Linked Enzyme Microcrystals of Subtilisin Carlsberg
Abstract We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic activity of CLECs in SC‐ethane was found to be 2‐ to 10‐fold greater than in hexane under the same conditions, using CLECs dried by propanol w...
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crwiley:10.1021/bp000148m 2024-06-02T08:05:11+00:00 Supercritical Fluids Are Superior Media for Catalysis by Cross‐Linked Enzyme Microcrystals of Subtilisin Carlsberg Fontes, Nuno Almeida, M. Conceição Garcia, Silvia Peres, Célia Partridge, Johann Halling, Peter J. Barreiros, Susana 2001 http://dx.doi.org/10.1021/bp000148m https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1021%2Fbp000148m https://onlinelibrary.wiley.com/doi/full/10.1021/bp000148m en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biotechnology Progress volume 17, issue 2, page 355-358 ISSN 8756-7938 1520-6033 journal-article 2001 crwiley https://doi.org/10.1021/bp000148m 2024-05-03T11:31:19Z Abstract We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic activity of CLECs in SC‐ethane was found to be 2‐ to 10‐fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air‐dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC‐CO 2 , especially at higher water activity, probably as a result of acid‐base effects of carbonic acid on the enzyme. Article in Journal/Newspaper Carbonic acid Wiley Online Library Biotechnology Progress 17 2 355 358 |
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Wiley Online Library |
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English |
description |
Abstract We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic activity of CLECs in SC‐ethane was found to be 2‐ to 10‐fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air‐dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC‐CO 2 , especially at higher water activity, probably as a result of acid‐base effects of carbonic acid on the enzyme. |
format |
Article in Journal/Newspaper |
author |
Fontes, Nuno Almeida, M. Conceição Garcia, Silvia Peres, Célia Partridge, Johann Halling, Peter J. Barreiros, Susana |
spellingShingle |
Fontes, Nuno Almeida, M. Conceição Garcia, Silvia Peres, Célia Partridge, Johann Halling, Peter J. Barreiros, Susana Supercritical Fluids Are Superior Media for Catalysis by Cross‐Linked Enzyme Microcrystals of Subtilisin Carlsberg |
author_facet |
Fontes, Nuno Almeida, M. Conceição Garcia, Silvia Peres, Célia Partridge, Johann Halling, Peter J. Barreiros, Susana |
author_sort |
Fontes, Nuno |
title |
Supercritical Fluids Are Superior Media for Catalysis by Cross‐Linked Enzyme Microcrystals of Subtilisin Carlsberg |
title_short |
Supercritical Fluids Are Superior Media for Catalysis by Cross‐Linked Enzyme Microcrystals of Subtilisin Carlsberg |
title_full |
Supercritical Fluids Are Superior Media for Catalysis by Cross‐Linked Enzyme Microcrystals of Subtilisin Carlsberg |
title_fullStr |
Supercritical Fluids Are Superior Media for Catalysis by Cross‐Linked Enzyme Microcrystals of Subtilisin Carlsberg |
title_full_unstemmed |
Supercritical Fluids Are Superior Media for Catalysis by Cross‐Linked Enzyme Microcrystals of Subtilisin Carlsberg |
title_sort |
supercritical fluids are superior media for catalysis by cross‐linked enzyme microcrystals of subtilisin carlsberg |
publisher |
Wiley |
publishDate |
2001 |
url |
http://dx.doi.org/10.1021/bp000148m https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1021%2Fbp000148m https://onlinelibrary.wiley.com/doi/full/10.1021/bp000148m |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_source |
Biotechnology Progress volume 17, issue 2, page 355-358 ISSN 8756-7938 1520-6033 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1021/bp000148m |
container_title |
Biotechnology Progress |
container_volume |
17 |
container_issue |
2 |
container_start_page |
355 |
op_container_end_page |
358 |
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1800749962232856576 |