Supercritical Fluids Are Superior Media for Catalysis by Cross‐Linked Enzyme Microcrystals of Subtilisin Carlsberg

Abstract We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic activity of CLECs in SC‐ethane was found to be 2‐ to 10‐fold greater than in hexane under the same conditions, using CLECs dried by propanol w...

Full description

Bibliographic Details
Published in:Biotechnology Progress
Main Authors: Fontes, Nuno, Almeida, M. Conceição, Garcia, Silvia, Peres, Célia, Partridge, Johann, Halling, Peter J., Barreiros, Susana
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2001
Subjects:
Carbonic acid
Online Access:http://dx.doi.org/10.1021/bp000148m
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1021%2Fbp000148m
https://onlinelibrary.wiley.com/doi/full/10.1021/bp000148m
Description
Summary:Abstract We report on the performance of cross‐linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC‐fluids). The catalytic activity of CLECs in SC‐ethane was found to be 2‐ to 10‐fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air‐dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC‐CO 2 , especially at higher water activity, probably as a result of acid‐base effects of carbonic acid on the enzyme.

Similar Items