Enzymatic production and physicochemical characterization of uncommon wax esters and monoglycerides

Abstract Wax esters from fatty alcohols and uncommon fatty acids were synthesized in yields up to 90% when commercially available microbial lipases from Rhizomucor miehei (Lipozyme ™ ) and Candida antarctica (SP 435) were used with limited water content in nonpolar solvents under mild conditions. Th...

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Bibliographic Details
Published in:Journal of the American Oil Chemists' Society
Main Authors: Multzsch, Raimund, Lokotsch, Wolfram, Steffen, Beate, Lang, Siegmund, Metzger, Jürgen O., Schäfer, Hans J., Warwel, Siegfried, Wagner, Fritz
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 1994
Subjects:
Brewster
Langmuir
Antarc*
Antarctica
Online Access:http://dx.doi.org/10.1007/bf02541428
https://onlinelibrary.wiley.com/doi/full/10.1007/BF02541428
Description
Summary:Abstract Wax esters from fatty alcohols and uncommon fatty acids were synthesized in yields up to 90% when commercially available microbial lipases from Rhizomucor miehei (Lipozyme ™ ) and Candida antarctica (SP 435) were used with limited water content in nonpolar solvents under mild conditions. The corresponding fatty acids were prepared by chemical conversion of naturally occurring resources (agricultural surpluses). Also, when phenylboronic acid was added as solubilizing agent in a nonpolar solvent, the direct enzymatic monoacylation of glycerol with uncommon fatty acids was successful. The measurement of π/A‐isotherms by means of a Langmuir film balance indicated medium film pressures, medium or large molecular areas, and interesting phase behavior. The monolayer of a wax ester at the air/water interface could be directly visualized by Brewster angle microscopy.

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