Molecular species of 1‐ O‐alk‐1′‐enyl‐2‐acyl‐, 1‐ O‐alkyl‐2‐acyl‐ and 1,2‐diacyl glycerophospholipids in Japanese oyster Crassostrea gigas (Thunberg)

• Published in: Lipids
• Main Authors: Yeong, Bo Young, Ohshima, Toshiaki, Koizumi, Chiaki
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 1990
• Subjects: Crassostrea gigas
• Online Access: http://dx.doi.org/10.1007/bf02536013; https://onlinelibrary.wiley.com/doi/full/10.1007/BF02536013

Abstract Molecular species of 1‐ O ‐alk‐1′‐enyl‐2‐acyl‐, 1‐ O ‐alkyl‐2‐acyl‐, and 1,2‐diacyl‐ sn ‐glycero‐3‐phosphoethanolamine (EPL) and sn ‐glycero‐3‐phosphocholine (CPL) of Japanese oyster Crassostrea gigas were analyzed by selectedion monitoring gas chromatography/mass spectrometry using electron impact ionization. The characteristic fragment ions, [RCH=CH+56] + due to the alkenyl residue in the sn ‐1 position and [RCO+74] + due to the acyl residue in the sn ‐2 position of alkenylacylglycerols, [R+130] + due to the alkyl residue in the sn ‐1 position and [RCO+74] + due to the acyl residue in the sn ‐2 position of alkylacylglycerols, [RCO+74] + due to the acyl residues in the sn ‐1 and/or sn ‐2 positions of diacylglycerols, and [M−57] + being indicative of the corresponding molecular weight, were used for structural assignments. For alkenylacyl EPL and CPL, 19 and 16 molecular species were determined, respectively. Two molecular species, 18∶0alkenyl‐22∶6n−3 and 18∶0‐alkenyl‐22∶2‐non‐methylene interrupted diene (NMID), amounted to 53.2% and 47.9%, respectively. The alkylacyl EPL and CPL consisted of 16 and 20 molecular species, respectively, and the prominent components were 18∶0alkyl‐22∶2NMID, 20∶1alkyl‐20∶1n−11 (27.4%) and 20∶1alkyl‐20∶2NMID (16.3%) in the former, and 16∶0alkyl‐20∶5n−3 (23.0%) and 16∶0alkyl‐22∶6n−3 (21.6%) in the latter. For the diacyl EPL and CPL, 14 and 51 molecular species were determined, respectively. The major molecular species were 18∶0–20∶5n−3 (37.4%), 16∶0–20∶5n−3 (14.2%) and 18∶1n−7–22∶2NMID (13.2%) in the former, and 16∶0–20∶5n−3 (33.4%) and 16∶0–22∶6n−3 (22.3%) in the latter. It was found that there were significant differences in the molecular species between the alkylacyl and diacyl EPL and the alkylacyl and diacyl CPL; the number of molecular species was larger in CPL than in EPL, while the number of total carbons and double bonds of the major molecular species were larger in the EPL than in the CPL. Alkenylacyl EPL were similar to alkenylacyl CPL in molecular species ...