Molecular species analysis of phosphoglycerides from the ripe roes of cod ( Gadus morhua)
Abstract Molecular species of the 3,5‐dinitrobenzoyl derivatives of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) were quantitated by UV detection at 254 nm after reversed‐phase HPLC using solvent systems modified from Takamura et al. ( Lipids 21 , 356–361, 19...
| Published in: | Lipids |
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| Main Author: | |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wiley
1989
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1007/bf02535073 https://onlinelibrary.wiley.com/doi/full/10.1007/BF02535073 |
| Summary: | Abstract Molecular species of the 3,5‐dinitrobenzoyl derivatives of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) were quantitated by UV detection at 254 nm after reversed‐phase HPLC using solvent systems modified from Takamura et al. ( Lipids 21 , 356–361, 1986). Three isocratic solvent systems were used and a total of 39 different molecular species detected. Four species, 16∶0/20∶5, 18∶1/20∶5, 16∶0/22∶6 and 18∶1/22∶6 contributed 67.2% and 61.8% of PC and PE respectively but only 23.0% of PI. In PI the most important species was 18∶0/20∶4 at 36.7% but this species only constituted 0.7% in each of PC and PE. Small amounts of dipolyunsaturated species were also found in PC and PE. |
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