Atmospheric pressure chemical ionisation liquid chromatography/multistage mass spectrometry for assignment of sedimentary bacteriochlorophyll derivatives
Abstract Atmospheric pressure chemical ionisation liquid chromatography/multistage mass spectrometry (APCI‐LC/MS n ) provides a rapid, on‐line method for the assignment of individual bacteriophaeophorbide c and d methyl esters (BPMEs) in complex mixtures. The MS 2 spectrum for each component is diag...
| Published in: | Rapid Communications in Mass Spectrometry |
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| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wiley
2004
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1002/rcm.1749 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.1749 https://onlinelibrary.wiley.com/doi/full/10.1002/rcm.1749 |
| Summary: | Abstract Atmospheric pressure chemical ionisation liquid chromatography/multistage mass spectrometry (APCI‐LC/MS n ) provides a rapid, on‐line method for the assignment of individual bacteriophaeophorbide c and d methyl esters (BPMEs) in complex mixtures. The MS 2 spectrum for each component is diagnostic of the type of BPME ( c or d ), and characteristic losses in MS 5 and MS 6 permit assignment of the alkyl substituents at positions C‐8 and C‐12 of the macrocycle. MS 5 mass chromatograms permit the deconvolution of coeluting isobaric BPMEs, revealing the true profiles of the individual components. The distributions are different in lake sediments from la Salada de Chiprana (Spain) and Kirisjes Pond (Antarctica), and a novel BPME c with a neo ‐pentyl substituent has been observed in the Kirisjes Pond sediment. Copyright © 2004 John Wiley & Sons, Ltd. |
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