Potential Function of 3,5‐Dihydroxy‐4‐Methoxybenzyl Alcohol from Pacific Oyster ( Crassostrea gigas) in Brain of Old Mice

Scope 3,5‐Dihydroxy‐4‐methoxybenzyl alcohol (DHMBA) is found in oyster extracts in recent years and is reported to have antioxidant activity. Although it has been reported to be protective in various models of oxidative stress, the therapeutic effect of DHMBA on neurological damage caused by aging r...

Full description

Bibliographic Details
Published in:Molecular Nutrition & Food Research
Main Authors: Chen, MinYu, Liu, Min, Chen, JingHong, Liu, Xinwei, Tang, LiWei, Wang, Chao, Yu, Ziniu, Zhang, Yang, Tian, Jing
Other Authors: Shenzhen Fundamental Research Program, National Natural Science Foundation of China
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2024
Subjects:
Pacific
Crassostrea gigas
Pacific oyster
Online Access:http://dx.doi.org/10.1002/mnfr.202300469
https://onlinelibrary.wiley.com/doi/pdf/10.1002/mnfr.202300469
Description
Summary:Scope 3,5‐Dihydroxy‐4‐methoxybenzyl alcohol (DHMBA) is found in oyster extracts in recent years and is reported to have antioxidant activity. Although it has been reported to be protective in various models of oxidative stress, the therapeutic effect of DHMBA on neurological damage caused by aging remains to be demonstrated. Methods and Results The present study investigates the potential functions of DHMBA in brain of old C57BL/6J mice and aging cell model. Administration of DHMBA improves working memory, reduces anxiety behavior, decreases the expression levels of cell cycle proteins, cycin‐dependent kinase inhibitor 1(P21) and peptidase inhibitor 16(P16) and inhibits neuronal loss in old mice. The data obtained from the aging cell model are consistent with those from the old mice. The interaction between DHMBA and Kelch‐like ECH‐associated protein 1 (Keap1) is predicted by molecular docking assay, and then it is verified by co‐immunopricipitation (CoIP) that factor red lineage 2‐related factor 2 (Nrf2)‐Keap1 protein‐protein interaction is inhibited by DHMBA. Protein levels of Nrf2 and its target genes, such as glutathione peroxidase 4(GPX4) and heme oxygenase 1 (HO‐1), are detected in old mice and aging cell model. Conclusion This study provides new evidence that explores the antioxidant mechanism of DHMBA and implies a potential role of DHMBA on antiaging in brain.

Similar Items