Effect of Candida antarctica Lipase B Immobilization on the Porous Structure of the Carrier
Abstract A series of poly(GMA ‐co‐ EGDMA) resins with identical composition but varying particle sizes, pore radii, specific surface areas and specific volumes are studied to assess how Candida antarctica lipase B immobilization affects the porosity of the copolymer particles. Mercury porosimetry re...
| Published in: | Macromolecular Bioscience |
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| Main Authors: | , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wiley
2011
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1002/mabi.201100127 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmabi.201100127 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mabi.201100127 |
| Summary: | Abstract A series of poly(GMA ‐co‐ EGDMA) resins with identical composition but varying particle sizes, pore radii, specific surface areas and specific volumes are studied to assess how Candida antarctica lipase B immobilization affects the porosity of the copolymer particles. Mercury porosimetry reveals a significant change in the average pore size (up to 6.1‐fold), the specific surface area (up to 3.2‐fold) and the specific volume (up to 2.1‐fold) of the epoxy resin. A similar behaviour is observed for glutaraldehyde‐modified epoxy resins. The influences of the resin porosity properties on the loading of Candida antarctica lipase B during immobilization and on the hydrolytic activity (hydrolysis of p ‐nitrophenyl acetate) of the immobilized lipase are studied. magnified image |
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