Phytoplankton primary productivity: A dual‐incubation approach for direct comparison of photosystem II photosynthetic flux (JV PII ) and 14 C‐fixation experiments

• Published in: Limnology and Oceanography: Methods
• Main Authors: Schuback, Nina, Oxborough, Kevin, Burkitt‐Gray, Mary, López‐García, Patricia, Patey, Matthew D., Hammermeister, Emily, Wright, Alan, Moore, C. Mark
• Other Authors: National Centre for Earth Observation
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2024
• Subjects: Northeast Atlantic
• Online Access: http://dx.doi.org/10.1002/lom3.10635; https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lom3.10635

Abstract Singe‐turnover active chlorophyll a fluorometry (STAF) can be used to assess phytoplankton photosynthetic rates in terms of the photosystem II photochemical flux (JV PII , μ mol e − m −3 s −1 ) instantaneously, autonomously, and at high resolution. While JV PII provides an upper limit to rates of phytoplankton primary productivity in units of carbon fixation, the conversion between these two rates is variable, limiting our ability to utilize high‐resolution JV PII data to monitor phytoplankton primary productivity. Simultaneous measurements of JV PII and 14 C‐fixation help in understanding the factors controlling the variable ratio between the two rates. However, to date, methodological inconsistencies, including differences in incubation lengths and light quality, have greatly inhibited practical assessment of such electron to carbon ratios (Φ e,C , mol e − mol C −1 ). We here present data from a range of dual‐incubation experiments in northeast Atlantic waters during which JV PII and 14 C‐fixation were measured simultaneously on the same sample. Time‐course experiments show how Φ e,C increases with incubation length, likely reflecting the transition from gross to net 14 C‐fixation. Dual‐incubation experiments conducted under different light levels show a tendency for increased Φ e,C under (super‐)saturating light. Finally, data from a diurnal experiment demonstrate how Φ e,C increases over the course of a day, due to downregulation of 14 C‐fixation. We provide a detailed description of our methodological approach, including a critical discussion of improvements to the calculation of JV PII implemented in the LabSTAF instrument used for active fluorescence measurements and the limitations of the well‐established 14 C‐fixation approach.