Absolute quantification of Atlantic salmon and rainbow trout vitellogenin by the ‘signature peptide’ approach using electrospray ionization QqToF tandem mass spectrometry

• Published in: Journal of Mass Spectrometry
• Main Authors: Cohen, Alejandro M., Mansour, Atef A. H., Banoub, Joseph H.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2006
• Subjects: Atlantic salmon
• Online Access: http://dx.doi.org/10.1002/jms.1023; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjms.1023; https://onlinelibrary.wiley.com/doi/full/10.1002/jms.1023

Abstract In this work, we present a simple method for absolute quantification of plasma vitellogenin from both rainbow trout and Atlantic salmon. Plasma samples obtained from control and β‐estradiol induced fish were digested with trypsin. A characteristic ‘signature peptide’ was selected and analyzed by high performance liquid chromatography (HPLC) coupled to an electrospray quadrupole‐time‐of‐flight tandem mass spectrometer, using a deuterated homolog peptide as an internal standard. The hybrid tandem mass spectrometer was operated in a ‘pseudo’ selected reaction‐monitoring mode in which three diagnostic product ions were monitored for identification and quantification purposes. The reproducibility (coefficient of variation ∼5%) and sensitivity (limit of quantification (LOQ) of 0.009 mg/ml) achieved by this simple assay allow it to be considered as an alternative to immunological assays. Copyright © 2006 John Wiley & Sons, Ltd.