Absolute quantification of Atlantic salmon and rainbow trout vitellogenin by the ‘signature peptide’ approach using electrospray ionization QqToF tandem mass spectrometry
Abstract In this work, we present a simple method for absolute quantification of plasma vitellogenin from both rainbow trout and Atlantic salmon. Plasma samples obtained from control and β‐estradiol induced fish were digested with trypsin. A characteristic ‘signature peptide’ was selected and analyz...
| Published in: | Journal of Mass Spectrometry |
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| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wiley
2006
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1002/jms.1023 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjms.1023 https://onlinelibrary.wiley.com/doi/full/10.1002/jms.1023 |
| Summary: | Abstract In this work, we present a simple method for absolute quantification of plasma vitellogenin from both rainbow trout and Atlantic salmon. Plasma samples obtained from control and β‐estradiol induced fish were digested with trypsin. A characteristic ‘signature peptide’ was selected and analyzed by high performance liquid chromatography (HPLC) coupled to an electrospray quadrupole‐time‐of‐flight tandem mass spectrometer, using a deuterated homolog peptide as an internal standard. The hybrid tandem mass spectrometer was operated in a ‘pseudo’ selected reaction‐monitoring mode in which three diagnostic product ions were monitored for identification and quantification purposes. The reproducibility (coefficient of variation ∼5%) and sensitivity (limit of quantification (LOQ) of 0.009 mg/ml) achieved by this simple assay allow it to be considered as an alternative to immunological assays. Copyright © 2006 John Wiley & Sons, Ltd. |
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