Shipboard analytical flow cytometry of oceanic ultraphytoplankton

Abstract Shipboard analysis of marine ultraphytoplankton by flow cytometry is a powerful method to classify these cells according to in vivo fluorescence characteristics and size. At present, this ataxonomic‐allometric approach allows recognition of phycoerythrin‐containing cyanobacteria, cryptomona...

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Bibliographic Details
Published in:Cytometry
Main Author: Li, William K. W.
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 1989
Subjects:
Online Access:http://dx.doi.org/10.1002/cyto.990100512
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcyto.990100512
https://onlinelibrary.wiley.com/doi/pdf/10.1002/cyto.990100512
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Summary:Abstract Shipboard analysis of marine ultraphytoplankton by flow cytometry is a powerful method to classify these cells according to in vivo fluorescence characteristics and size. At present, this ataxonomic‐allometric approach allows recognition of phycoerythrin‐containing cyanobacteria, cryptomonads, very small red‐fluorescing cells (presumably prochlorophytes), and eukaryotic algae of various sizes in many open ocean samples. The speed at which flow cytometric analysis can be performed on freshly collected samples permits a high degree of sampling resolution in both space and time. A flow cytometric view is presented of the vertical distribution of ultraphytoplankton at various sites in the north Atlantic and of experiments wherein phytoplankton were incubated in an artificial light gradient and under simulated in situ conditions.