Plasma‐Modified Polypropylene as Carrier for the Immobilization of Candida antarctica Lipase B and Pyrobaculum calidifontis Esterase
Abstract In this work, the immobilization of two hydrolases on plasma‐modified polypropylene carriers was investigated. Treating Accurel MP1001 with an oxygen plasma was found most suitable to increase the hydrophilicity and to allow for efficient immobilization. Thus, for lipase B from Candida anta...
| Published in: | ChemCatChem |
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| Main Authors: | , , , , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wiley
2010
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1002/cctc.201000130 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcctc.201000130 http://onlinelibrary.wiley.com/wol1/doi/10.1002/cctc.201000130/fullpdf |
| Summary: | Abstract In this work, the immobilization of two hydrolases on plasma‐modified polypropylene carriers was investigated. Treating Accurel MP1001 with an oxygen plasma was found most suitable to increase the hydrophilicity and to allow for efficient immobilization. Thus, for lipase B from Candida antarctica and for an esterase from Pyrobaculum calidifontis esterase (PestE) a 5‐fold and 14‐fold increase, respectively, in immobilization yield resulted compared to untreated carrier. In contrast to the oxygen‐modified support, modification of the polypropylene carrier with ammonia plasma showed no positive effect. Furthermore, it could be shown that immobilized PestE catalyzed enantioselective transesterification of α‐phenylethanol in vinyl acetate, whereas the free enzyme showed no activity. Both hydrolases could be recycled five times without significant loss of activity. |
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