Rational Design of Pseudozyma antarctica Lipase B Yielding a General Esterification Catalyst

Abstract Pseudozyma antarctica lipase B (CALB) shows activity in the acrylation of hydroxypropylcarbamate, a racemic mixture of enantiomers of primary and secondary alcohols. However, full conversion is hampered by the slowly reacting S enantiomer of the secondary alcohol. The same is true for a wid...

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Bibliographic Details
Published in:ChemBioChem
Main Authors: Liu, Danni, Trodler, Peter, Eiben, Sabine, Koschorreck, Katja, Müller, Monika, Pleiss, Jürgen, Maurer, Steffen C., Branneby, Cecilia, Schmid, Rolf D., Hauer, Bernhard
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2010
Subjects:
Antarc*
Antarctica
Online Access:http://dx.doi.org/10.1002/cbic.200900776
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcbic.200900776
http://onlinelibrary.wiley.com/wol1/doi/10.1002/cbic.200900776/fullpdf
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Summary:Abstract Pseudozyma antarctica lipase B (CALB) shows activity in the acrylation of hydroxypropylcarbamate, a racemic mixture of enantiomers of primary and secondary alcohols. However, full conversion is hampered by the slowly reacting S enantiomer of the secondary alcohol. The same is true for a wide range of secondary alcohols, for example, octan‐2‐ and ‐3‐ol. In order to get high conversion in these reactions in a short time, the stereospecificity pocket of CALB was redesigned by using predictions from molecular modeling. Positions 278, 104, and 47 were targeted, and a library for two‐site saturation mutagenesis at positions 104 and 278 was constructed. The library was then screened for hydrolysis of acrylated hydroxypropylcarbamates. The best mutants L278A, L278V, L278A/W104F, and L278A/W104F/S47A showed an increased conversion in hydrolysis and transesterification of more than 30 %. While the wild‐type showed only 73 % conversion in the acrylation of hydroxypropylcarbamate after 6 h, 97 % conversion was achieved by L278A in this time. Besides this, L278A/W104F reached >96 % conversion in the acrylation of octan‐2‐ and ‐3‐ol within 48 h and showed a significant decrease in stereoselectivity, while the wild‐type reached only 68 and 59 % conversion, respectively. Thus the new biocatalysts can be used for efficient transformation of racemic alcohols and esters with high activity when the high stereoselectivity of the wild‐type hampers complete conversion of racemic substrates in a short time.

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