Influence of water activity on the enantioselective esterification of (R,S)‐ibuprofen by crosslinked crystals of Candida antarctica lipase B in organic solvent media
Abstract The lipase B from Candida antarctica was purified from a commercial source and crystalized. The microcrystals were crosslinked using glutaraldehyde. The crosslinked crystals were then used to catalyze the esterification of ( R,S )‐ibuprofen with dodecanol in octane at various water activiti...
| Published in: | Biotechnology and Bioengineering |
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| Main Authors: | , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wiley
2001
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1002/bit.10051 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.10051 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.10051 |
| Summary: | Abstract The lipase B from Candida antarctica was purified from a commercial source and crystalized. The microcrystals were crosslinked using glutaraldehyde. The crosslinked crystals were then used to catalyze the esterification of ( R,S )‐ibuprofen with dodecanol in octane at various water activities. As for the commercial preparation immobilized on acrylic resin, Novozym 435, low water activities foster better enantioselectivity, and the maximum reaction rates are obtained for a W = 0.1. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 75: 559–562, 2001. |
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