Development and characterization of thirteen novel microsatellite markers for use in Greenland sharks (Somniosus microcephalus), with cross-amplification in Pacific sleeper sharks (Somniosus pacificus)

• Published in: BMC Research Notes
• Main Authors: Swintek, Meaghan A., Walter, Ryan P.
• Other Authors: California State University, Fullerton
• Format: Article in Journal/Newspaper
• Language: English
• Published: Springer Science and Business Media LLC 2021
• Subjects: General Biochemistry, Genetics and Molecular Biology; General Medicine; Greenland; Pacific; Svalbard; Somniosus microcephalus
• Online Access: http://dx.doi.org/10.1186/s13104-021-05447-5; http://link.springer.com/content/pdf/10.1186/s13104-021-05447-5.pdf; http://link.springer.com/article/10.1186/s13104-021-05447-5/fulltext.html

Abstract Objective The objectives of this work are to isolate, develop, and characterize polymorphic microsatellite markers for use in Greenland sharks ( Somniosus microcephalus ). Results Thirteen microsatellite loci were successfully amplified and yielded multi-locus genotypes for 36 S. microcephalus individuals from Grise Fjord (n = 16) and Svalbard (n = 20). Each locus yielded between 2 and 9 alleles and observed heterozygosity ranged from 0.11 to 0.70 when estimated across both sites. One locus and three loci deviated from HWE following Bonferroni correction, for individuals sampled from Grise Fjord and Svalbard, respectively. Cross-amplification was successful at every locus for five of the ten S. pacificus individuals.