Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon

• Published in: Scientific Reports
• Main Authors: Güralp, Hilal, Skaftnesmo, Kai O., Kjærner-Semb, Erik, Straume, Anne Hege, Kleppe, Lene, Schulz, Rüdiger W., Edvardsen, Rolf B., Wargelius, Anna
• Other Authors: Norges Forskningsråd
• Format: Article in Journal/Newspaper
• Language: English
• Published: Springer Science and Business Media LLC 2020
• Subjects: Multidisciplinary; Atlantic salmon; Salmo salar
• Online Access: http://dx.doi.org/10.1038/s41598-020-74876-2; https://www.nature.com/articles/s41598-020-74876-2.pdf; https://www.nature.com/articles/s41598-020-74876-2

Abstract Genetic introgression of escaped farmed Atlantic salmon ( Salmo salar ) into wild populations is a major environmental concern for the salmon aquaculture industry. Using sterile fish in commercial aquaculture operations is, therefore, a sustainable strategy for bio-containment. So far, the only commercially used methodology for producing sterile fish is triploidization. However, triploid fish are less robust. A novel approach in which to achieve sterility is to produce germ cell-free salmon, which can be accomplished by knocking out the dead-end ( dnd ) gene using CRISPR-Cas9. The lack of germ cells in the resulting dnd crispants, thus, prevents reproduction and inhibits subsequent large-scale production of sterile fish. Here, we report a rescue approach for producing germ cells in Atlantic salmon dnd crispants. To achieve this, we co-injected the wild-type (wt) variant of salmon dnd mRNA together with CRISPR-Cas9 constructs targeting dnd into 1-cell stage embryos. We found that rescued one-year-old fish contained germ cells, type A spermatogonia in males and previtellogenic primary oocytes in females. The method presented here opens a possibility for large-scale production of germ-cell free Atlantic salmon offspring through the genetically sterile broodstock which can pass the sterility trait on the next generation.