Induction of G-bands on Anguilla anguilla chromosomes by the restriction endonucleases Haelll, Hinf I, and Mse I

Fixed metaphase chromosomes of the eel Anguilla anguilla were treated with the restriction enzymes Haelll, Hinf I, and Mse I. All three restriction endonucleases are capable of inducing a G-band-like pattern if the incubation time and enzyme concentration are controlled. The results are discussed in...

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Bibliographic Details
Published in:Cytogenetic and Genome Research
Main Authors: Viñas, A., Gómez, C., Martínez, P., Sánchez, L.
Format: Article in Journal/Newspaper
Language:English
Published: S. Karger AG 1994
Subjects:
Anguilla anguilla
Online Access:http://dx.doi.org/10.1159/000133604
https://www.karger.com/Article/Pdf/133604
Description
Summary:Fixed metaphase chromosomes of the eel Anguilla anguilla were treated with the restriction enzymes Haelll, Hinf I, and Mse I. All three restriction endonucleases are capable of inducing a G-band-like pattern if the incubation time and enzyme concentration are controlled. The results are discussed in relation to the main factors involved in the appearance of serial G-bands. Chromatin organization, rather than DNA composition, seems to account for the interstitial G-band pattern exhibited by these eel chromosomes.

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