Evaluation of the performance of differently immobilized recombinant lipase B from Candida antarctica preparations for the synthesis of pharmacological derivatives in organic media

• Published in: RSC Advances
• Main Authors: Manoel, Evelin A., Robert, Julia M., Pinto, Martina C. C., Machado, Antonio C. O., Besteti, Marina D., Coelho, Maria Alice Z., Simas, Alessandro B. C., Fernandez-Lafuente, Roberto, Pinto, Jose Carlos, Freire, Denise M. G.
• Other Authors: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Conselho Nacional de Desenvolvimento Científico e Tecnológico
• Format: Article in Journal/Newspaper
• Language: English
• Published: Royal Society of Chemistry (RSC) 2016
• Subjects: Antarc*; Antarctica
• Online Access: http://dx.doi.org/10.1039/c5ra22508f; http://pubs.rsc.org/en/content/articlepdf/2016/RA/C5RA22508F

This paper shows the production of lipase B from Candida antarctica (LIPB) after cloning the gene that encoded it in Pichia pastoris using PGK as a constitutive promoter. The lipase was immobilized on different home-made supports for distinct reactions.