Evaluation of the performance of differently immobilized recombinant lipase B from Candida antarctica preparations for the synthesis of pharmacological derivatives in organic media

This paper shows the production of lipase B from Candida antarctica (LIPB) after cloning the gene that encoded it in Pichia pastoris using PGK as a constitutive promoter. The lipase was immobilized on different home-made supports for distinct reactions.

Bibliographic Details
Published in:RSC Advances
Main Authors: Manoel, Evelin A., Robert, Julia M., Pinto, Martina C. C., Machado, Antonio C. O., Besteti, Marina D., Coelho, Maria Alice Z., Simas, Alessandro B. C., Fernandez-Lafuente, Roberto, Pinto, Jose Carlos, Freire, Denise M. G.
Other Authors: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Conselho Nacional de Desenvolvimento Científico e Tecnológico
Format: Article in Journal/Newspaper
Language:English
Published: Royal Society of Chemistry (RSC) 2016
Subjects:
Antarc*
Antarctica
Online Access:http://dx.doi.org/10.1039/c5ra22508f
http://pubs.rsc.org/en/content/articlepdf/2016/RA/C5RA22508F
Description
Summary:This paper shows the production of lipase B from Candida antarctica (LIPB) after cloning the gene that encoded it in Pichia pastoris using PGK as a constitutive promoter. The lipase was immobilized on different home-made supports for distinct reactions.

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