Papaya ( Carica papaya ) lipase with some distinct acyl and alkyl specificities as compared with microbial lipases

• Published in: Biochemical Society Transactions
• Main Authors: Gandhi, N. N., Mukherjee, K. D.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Portland Press Ltd. 2000
• Subjects: Biochemistry; Antarc*; Antarctica
• Online Access: http://dx.doi.org/10.1042/bst0280977; https://portlandpress.com/biochemsoctrans/article-pdf/28/6/977/535359/bst0280977.pdf

Lipase from papaya (Carica papaya) latex (CPL), Candida antarctica lipase B (Novozym 435, NOV) and Rhizomucor miehei lipase (Lipozyme IM 20, LIP) were used as biocatalysts for the esterification of caprylic acid with straight-chain saturated C4-C18 alcohols and unsaturated C18 alcohols, such as cis-9-octadecenyl (oleyl, C18:1, n-9), cis-6-octadecenyl (petroselinyl, C18:1, n-12), cis-9, cis-12-octadecadienyl (linoleyl, C18:2, n-6), all-cis-9,12,15-octadecatrienyl (α-linolenyl, C18:3, n-3) and all-cis-6,9,12-octadecatrienyl (γ-linolenyl, C18:3, n-6) alcohols. With CPL, highest activity was found in the esterification of octanol and decanol, whereas both NOV and LIP showed a broad chain-length-specificity for the alcohols. CPL, as opposed to the microbial lipases, strongly discriminated against all the saturated long-chain (> C12 and unsaturated C18 alcohols.