Papaya ( Carica papaya ) lipase with some distinct acyl and alkyl specificities as compared with microbial lipases
Lipase from papaya (Carica papaya) latex (CPL), Candida antarctica lipase B (Novozym 435, NOV) and Rhizomucor miehei lipase (Lipozyme IM 20, LIP) were used as biocatalysts for the esterification of caprylic acid with straight-chain saturated C4-C18 alcohols and unsaturated C18 alcohols, such as cis-...
| Published in: | Biochemical Society Transactions |
|---|---|
| Main Authors: | , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Portland Press Ltd.
2000
|
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1042/bst0280977 https://portlandpress.com/biochemsoctrans/article-pdf/28/6/977/535359/bst0280977.pdf |
| Summary: | Lipase from papaya (Carica papaya) latex (CPL), Candida antarctica lipase B (Novozym 435, NOV) and Rhizomucor miehei lipase (Lipozyme IM 20, LIP) were used as biocatalysts for the esterification of caprylic acid with straight-chain saturated C4-C18 alcohols and unsaturated C18 alcohols, such as cis-9-octadecenyl (oleyl, C18:1, n-9), cis-6-octadecenyl (petroselinyl, C18:1, n-12), cis-9, cis-12-octadecadienyl (linoleyl, C18:2, n-6), all-cis-9,12,15-octadecatrienyl (α-linolenyl, C18:3, n-3) and all-cis-6,9,12-octadecatrienyl (γ-linolenyl, C18:3, n-6) alcohols. With CPL, highest activity was found in the esterification of octanol and decanol, whereas both NOV and LIP showed a broad chain-length-specificity for the alcohols. CPL, as opposed to the microbial lipases, strongly discriminated against all the saturated long-chain (> C12 and unsaturated C18 alcohols. |
|---|