Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm
Vibrio splendidus is a common pathogen in the ocean that infects Apostichopus japonicus , Atlantic salmo n and Crassostrea gigas , leading to a variety of diseases. In this study, a virulent phage vB_VspM_VS1, which infects V . splendidus , was isolated from aquaculture ponds in Dalian, China, and i...
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crplos:10.1371/journal.pone.0289895 2024-05-19T07:39:19+00:00 Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm Duan, Xuemei Jiang, Liming Guo, Ming Li, Chenghua Dinamarca, M. Alejandro National Natural Science Foundation of China Natural Science Foundation of Ningbo special Fund Project of Education Scientific and Technological Innovation of Gansu Province General project of Education Department of Zhejiang Province Science Foundation of Gansu Province of China Zhejiang Provincial Natural Science Foundation of China Ningbo Yongjiang Talent Introduction Programme the K.C. Wong Magna Fund at Ningbo University 2023 http://dx.doi.org/10.1371/journal.pone.0289895 https://dx.plos.org/10.1371/journal.pone.0289895 en eng Public Library of Science (PLoS) http://creativecommons.org/licenses/by/4.0/ PLOS ONE volume 18, issue 9, page e0289895 ISSN 1932-6203 journal-article 2023 crplos https://doi.org/10.1371/journal.pone.0289895 2024-05-01T07:06:19Z Vibrio splendidus is a common pathogen in the ocean that infects Apostichopus japonicus , Atlantic salmo n and Crassostrea gigas , leading to a variety of diseases. In this study, a virulent phage vB_VspM_VS1, which infects V . splendidus , was isolated from aquaculture ponds in Dalian, China, and it belongs to the family Straboviridae in the order Caudoviricetes . vB_VspM_VS1 had an adsorption rate of 96% in 15 min, a latent period of 65 min, and a burst size of 140 ± 6 PFU/cell. The complete genome of phage vB_VspM_VS1 consists of a linear double-stranded DNA that is 248,270 bp in length with an average G + C content of 42.5% and 389 putative protein-coding genes; 116 genes have known functions. There are 4 tail fiber genes in the positive and negative strands of the phage vB_VspM_VS1 genome. The protein domain of the phage vB_VspM_VS1 tail fibers was obtained from the Protein Data Bank and the SMART ( http://smart.embl.de ) database. Bacterial challenge tests revealed that the growth of V . splendidus HS0 was apparently inhibited (OD 600 < 0.01) in 12 h at an MOI of 10. In against biofilms, we also showed that the OD 570 value of the vB_VspM_VS1-treated group (MOI = 1) decreased significantly to 0.04 ± 0.01 compared with that of the control group (0.48 ± 0.08) at 24 h. This study characterizes the genome of the phage vB_VspM_VS1 that infects the pathogenic bacterium V . splendidus of A . japonicus . Article in Journal/Newspaper Crassostrea gigas PLOS PLOS ONE 18 9 e0289895 |
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English |
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Vibrio splendidus is a common pathogen in the ocean that infects Apostichopus japonicus , Atlantic salmo n and Crassostrea gigas , leading to a variety of diseases. In this study, a virulent phage vB_VspM_VS1, which infects V . splendidus , was isolated from aquaculture ponds in Dalian, China, and it belongs to the family Straboviridae in the order Caudoviricetes . vB_VspM_VS1 had an adsorption rate of 96% in 15 min, a latent period of 65 min, and a burst size of 140 ± 6 PFU/cell. The complete genome of phage vB_VspM_VS1 consists of a linear double-stranded DNA that is 248,270 bp in length with an average G + C content of 42.5% and 389 putative protein-coding genes; 116 genes have known functions. There are 4 tail fiber genes in the positive and negative strands of the phage vB_VspM_VS1 genome. The protein domain of the phage vB_VspM_VS1 tail fibers was obtained from the Protein Data Bank and the SMART ( http://smart.embl.de ) database. Bacterial challenge tests revealed that the growth of V . splendidus HS0 was apparently inhibited (OD 600 < 0.01) in 12 h at an MOI of 10. In against biofilms, we also showed that the OD 570 value of the vB_VspM_VS1-treated group (MOI = 1) decreased significantly to 0.04 ± 0.01 compared with that of the control group (0.48 ± 0.08) at 24 h. This study characterizes the genome of the phage vB_VspM_VS1 that infects the pathogenic bacterium V . splendidus of A . japonicus . |
author2 |
Dinamarca, M. Alejandro National Natural Science Foundation of China Natural Science Foundation of Ningbo special Fund Project of Education Scientific and Technological Innovation of Gansu Province General project of Education Department of Zhejiang Province Science Foundation of Gansu Province of China Zhejiang Provincial Natural Science Foundation of China Ningbo Yongjiang Talent Introduction Programme the K.C. Wong Magna Fund at Ningbo University |
format |
Article in Journal/Newspaper |
author |
Duan, Xuemei Jiang, Liming Guo, Ming Li, Chenghua |
spellingShingle |
Duan, Xuemei Jiang, Liming Guo, Ming Li, Chenghua Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm |
author_facet |
Duan, Xuemei Jiang, Liming Guo, Ming Li, Chenghua |
author_sort |
Duan, Xuemei |
title |
Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm |
title_short |
Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm |
title_full |
Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm |
title_fullStr |
Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm |
title_full_unstemmed |
Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm |
title_sort |
isolation, characterization and application of a lytic phage vb_vspm_vs1 against vibrio splendidus biofilm |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1371/journal.pone.0289895 https://dx.plos.org/10.1371/journal.pone.0289895 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
PLOS ONE volume 18, issue 9, page e0289895 ISSN 1932-6203 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1371/journal.pone.0289895 |
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