Isolation, characterization and application of a lytic phage vB_VspM_VS1 against Vibrio splendidus biofilm
Vibrio splendidus is a common pathogen in the ocean that infects Apostichopus japonicus , Atlantic salmo n and Crassostrea gigas , leading to a variety of diseases. In this study, a virulent phage vB_VspM_VS1, which infects V . splendidus , was isolated from aquaculture ponds in Dalian, China, and i...
Published in: | PLOS ONE |
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Main Authors: | , , , |
Other Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2023
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Subjects: | |
Online Access: | http://dx.doi.org/10.1371/journal.pone.0289895 https://dx.plos.org/10.1371/journal.pone.0289895 |
Summary: | Vibrio splendidus is a common pathogen in the ocean that infects Apostichopus japonicus , Atlantic salmo n and Crassostrea gigas , leading to a variety of diseases. In this study, a virulent phage vB_VspM_VS1, which infects V . splendidus , was isolated from aquaculture ponds in Dalian, China, and it belongs to the family Straboviridae in the order Caudoviricetes . vB_VspM_VS1 had an adsorption rate of 96% in 15 min, a latent period of 65 min, and a burst size of 140 ± 6 PFU/cell. The complete genome of phage vB_VspM_VS1 consists of a linear double-stranded DNA that is 248,270 bp in length with an average G + C content of 42.5% and 389 putative protein-coding genes; 116 genes have known functions. There are 4 tail fiber genes in the positive and negative strands of the phage vB_VspM_VS1 genome. The protein domain of the phage vB_VspM_VS1 tail fibers was obtained from the Protein Data Bank and the SMART ( http://smart.embl.de ) database. Bacterial challenge tests revealed that the growth of V . splendidus HS0 was apparently inhibited (OD 600 < 0.01) in 12 h at an MOI of 10. In against biofilms, we also showed that the OD 570 value of the vB_VspM_VS1-treated group (MOI = 1) decreased significantly to 0.04 ± 0.01 compared with that of the control group (0.48 ± 0.08) at 24 h. This study characterizes the genome of the phage vB_VspM_VS1 that infects the pathogenic bacterium V . splendidus of A . japonicus . |
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