Description and genome-wide analysis of Profundicola chukchiensis gen. nov., sp. nov., marine bacteria isolated from bottom sediments of the Chukchi Sea

• Published in: PLOS ONE
• Main Authors: Romanenko, Lyudmila, Otstavnykh, Nadezhda, Kurilenko, Valeriya, Velansky, Peter, Eremeev, Viacheslav, Mikhailov, Valery, Isaeva, Marina P.
• Other Authors: Maldonado Manjarrez, Luis A. Angel, Ministry of Science and Higher Education, Russian Federation
• Format: Article in Journal/Newspaper
• Language: English
• Published: Public Library of Science (PLoS) 2023
• Subjects: Arctic; Arctic Ocean; Chukchi; Chukchi Sea
• Online Access: http://dx.doi.org/10.1371/journal.pone.0287346; https://dx.plos.org/10.1371/journal.pone.0287346

Two Gram-negative, aerobic halophilic non-motile strains designated KMM 9713 and KMM 9724 T were isolated from the bottom sediments sampled from the Chukchi Sea in the Arctic Ocean, Russia. The novel strains grew in 0.5−5% NaCl, at 7−42°C, and pH 5.5−10.5. Phylogenetic analyses based on 16S rRNA gene and whole genome sequences revealed that strains KMM 9713 and KMM 9724 T were close to each other and shared the highest 16S rRNA gene sequence similarity of 91.28% with the type strain Ornithobacterium rhinotracheale DSM 15997 T and 90.15–90.92% with the members of the genus Empedobacter in the family Weeksellaceae . Phylogenetic trees indicated that strains KMM 9713 and KMM 9724 T formed a distinct line adjacent to their relative O . rhinotracheale DSM 15997 T . The average nucleotide identity values between strain KMM 9724 T and O . rhinotracheale DSM 15997 T , Empedobacter brevis NBRC 14943 T , and Moheibacter sediminis CGMCC 1.12708 T were 76.73%, 75.78%, and 74.65%, respectively. The novel strains contained the predominant menaquinone MK-6 and the major fatty acids of iso-C 17:0 3-OH, iso-C 15:0 followed by iso-C 17:1 ω 6. Polar lipids consisted of phosphatidylethanolamine, one an unidentified aminophospholipid, two unidentified aminolipids, and two or three unidentified lipids. The DNA G+C contents of 34.5% and 34.7% were calculated from genome sequence of the strains KMM 9713 and KMM 9724 T , respectively. Based on the phylogenetic evidence and distinctive phenotypic characteristics, strains KMM 9713 and KMM 9724 T are proposed to be classified as a novel genus and species Profundicola chukchiensis gen. nov., sp. nov. The type strain of Profundicola chukchiensis gen. nov., sp. nov. is strain KMM 9724 T (= KACC 22806 T ).