Intrinsic post‐ejaculation sperm ageing does not affect offspring fitness in Atlantic salmon

• Published in: Journal of Evolutionary Biology
• Main Authors: Hotzy, Cosima, Xuhui, Bao, Larva, Tuuli, Immler, Simone
• Other Authors: H2020 European Research Council, Human Frontier Science Program, Knut och Alice Wallenbergs Stiftelse
• Format: Article in Journal/Newspaper
• Language: English
• Published: Oxford University Press (OUP) 2020
• Subjects: Ecology, Evolution, Behavior and Systematics; Atlantic salmon; Salmo salar
• Online Access: http://dx.doi.org/10.1111/jeb.13590; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjeb.13590; https://onlinelibrary.wiley.com/doi/pdf/10.1111/jeb.13590; https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jeb.13590

Abstract Post‐meiotic sperm ageing, both before ejaculation and after ejaculation, has been shown to negatively affect offspring fitness by lowering the rate of embryonic development, reducing embryonic viability and decreasing offspring condition. These negative effects are thought to be caused by intrinsic factors such as oxidative stress and ATP depletion or extrinsic factors such as temperature and osmosis. Effects of post‐ejaculation sperm ageing on offspring fitness have so far almost exclusively been tested in internal fertilizers. Here, we tested whether intrinsic post‐ejaculation sperm ageing affects offspring performance in an external fertilizer, the Atlantic salmon Salmo salar . We performed in vitro fertilizations with a split‐clutch design where sperm were subjected to four post‐ejaculation ageing treatments. We varied the duration between sperm activation and fertilization while minimizing extrinsic stress factors and tested how this affected offspring fitness. We found no evidence for an effect of our treatments on embryo survival, hatching time, larval standard length, early larval survival or larval growth rate, indicating that intrinsic post‐ejaculation sperm ageing may not occur in Atlantic salmon. One reason may be the short life span of salmon sperm after ejaculation. Whether our findings are true in other external fertilizers with extended sperm activity remains to be tested.