Identification of a novel quinoline-based UV-protective pigment from a psychrotrophic Arctic bacterium

• Published in: Journal of Applied Microbiology
• Main Authors: Mandal, Sukhendu, Kundu, Shampa, Uddin, Md Raihan, Das, Prasenjit, Paul, Payel, Roy, Pranab, Tribedi, Prosun, Sahoo, Prithidipa
• Format: Article in Journal/Newspaper
• Language: English
• Published: Oxford University Press (OUP) 2022
• Subjects: Applied Microbiology and Biotechnology; General Medicine; Biotechnology; Arctic; Norway; Svalbard
• Online Access: http://dx.doi.org/10.1111/jam.15760; https://onlinelibrary.wiley.com/doi/pdf/10.1111/jam.15760; https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jam.15760; https://academic.oup.com/jambio/article-pdf/133/5/3059/48732526/jambio3059.pdf

Abstract Aims Psychrotrophs are extremophilic microorganisms that grow optimally in low temperature having many unique bioactive molecules of biotechnological applications. In this study, we characterized a pigment from an arctic bacterium with protective activity towards UV exposure. Methods and results The present research reports isolation and characterization of a psychrotrophic bacteria, RSAP2, from the soil sample of NyAlesund (78°56"N, 11°54"E), Svalbard, Norway. The strain showed closest 16S rRNA gene sequence similarity (99.9%) with Kocuria indica NIO-1021. RSAP2 is a Gram-positive, coccoid aerobe which produces a yellow pigment. The optimal parameters for pigment production while grown in LB medium were 3% (w/v) NaCl and 4 days of incubation of the culture at 20°C and pH 9 with shaking (180 rpm). The pigment was extracted in methanol and acetone (2:1) and further purified through column chromatography. It was characterized by mass spectrometry, UV–visible, fluorescence, IR, 1H NMR, 13C NMR spectroscopy and CHNS/O analysis. The pigment has a molecular weight of about 258 daltons and the molecular formula was determined as C15H18N2O2 and is a quinoline derivative. We show that the pigment can protect Escherichia coli against UV-mediated mutagenesis. We further demonstrate that the pigment displays a significant antimicrobial effect and in sublethal concentrations it impairs biofilm formation ability of the model organism Staphylococcus aureus. Conclusions The pigment of a psychrotrophic Arctic bacterium, most likely a strain of K. indica, was purified and its chemical structure was determined. The quinoline-based pigment has the ability to protect live cells from UV induced damage. Significance and impact of study Analysis and characterization of this newly isolated quinoline-based pigment is a potential candidate for future application in skin care products.