Simple Gas Chromatographic Method for the Quantification of Total Cholesterol in Fish Meats

Abstract Six fish speciesCirrhinus mrigala (Morakhi), Labeo rohita (Danbhro), Catla catla (Thalli), Wallago attu (Jarko), Ctenopharyngodon idella (Grass), and Cyprimus carpio (Gulfam)commonly found in the Pakistani Indus river, were selected for fat and cholesterol evaluation. Fat content was quanti...

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Bibliographic Details
Published in:Journal of AOAC INTERNATIONAL
Main Authors: Memon, Nusrat N, Sherazi, Syed Tufail H, Talpur, Farah N, Bhanger, Muhammad I
Format: Article in Journal/Newspaper
Language:English
Published: Oxford University Press (OUP) 2010
Subjects:
Pharmacology
Agronomy and Crop Science
Environmental Chemistry
Food Science
Analytical Chemistry
Attu
Online Access:http://dx.doi.org/10.1093/jaoac/93.4.1249
http://academic.oup.com/jaoac/article-pdf/93/4/1249/32422244/jaoac1249.pdf
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Summary:Abstract Six fish speciesCirrhinus mrigala (Morakhi), Labeo rohita (Danbhro), Catla catla (Thalli), Wallago attu (Jarko), Ctenopharyngodon idella (Grass), and Cyprimus carpio (Gulfam)commonly found in the Pakistani Indus river, were selected for fat and cholesterol evaluation. Fat content was quantified by the Folch method and cholesterol content was determined by a simple GC method. The application of the proposed method to quantify cholesterol content revealed variations among the six breeds evaluated. The breeds studied contained 0.801.95 fat, indicating that all investigated fishes were lean; significant variation was found in cholesterol content, and ranged between 72 and 392 mg/100 g. Standard addition analyses showed that the method was accurate, as the recovery of cholesterol varied from 95 to 103, with a coefficient of variance not more than 5.6. The results showed that, after the development of suitable calibration, within 11 min, the cholesterol could be accurately determined by GC.

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