Liquid Chromatographic Determination of Sulfadiazine in Salmon by Postcolumn Derivatization and Fluorescence Detection

Abstract A reversed-phase (ODS-2) liquid chromatographic method was developed to determine low nanogramper-gram levels of sulfadiazine (SDZ) in salmon muscle tissue. SDZ was extracted with acetonitrile-aqueous 2% acetic acid (pH 3.0), partitioned into methylene chloride, and cleaned up by using a st...

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Bibliographic Details
Published in:Journal of AOAC INTERNATIONAL
Main Authors: Gehring, Theresa A, Rushing, Larry G, Thompson, Harold C
Format: Article in Journal/Newspaper
Language:English
Published: Oxford University Press (OUP) 1995
Subjects:
Pharmacology
Agronomy and Crop Science
Environmental Chemistry
Food Science
Analytical Chemistry
Atlantic salmon
Online Access:http://dx.doi.org/10.1093/jaoac/78.5.1161
http://academic.oup.com/jaoac/article-pdf/78/5/1161/32456768/jaoca1161.pdf
Description
Summary:Abstract A reversed-phase (ODS-2) liquid chromatographic method was developed to determine low nanogramper-gram levels of sulfadiazine (SDZ) in salmon muscle tissue. SDZ was extracted with acetonitrile-aqueous 2% acetic acid (pH 3.0), partitioned into methylene chloride, and cleaned up by using a strong-cation-exchange, solid-phase extraction cartridge. SDZ was derivatized postcolumn with fluo-rescamine and detected by fluorescence. The limit of detection was 0.2 ng SDZ/g tissue. Recoveries from coho salmon tissue fortified with 1,5,10, and 20 ng SDZ/g tissue averaged 84.5,85.0,83.6, and 83.9%, respectively; recoveries from Atlantic salmon tissue fortified with 10 ng SDZ/g tissue averaged 82.6%.

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