Isolation of human pathogen Escherichia albertii from faeces of seals (Leptonychotes weddelli) in James Ross Island, Antarctica
A set of nine gram-negative fermenting rods biochemically identified as Escherichia coli was isolated from faeces of seals. These bacteria were characterized by phenotypic classification, 16S rDNA sequence analyses, automated ribotyping, study of whole-cell protein profiles by SDS-PAGE and finally b...
| Published in: | Czech Polar Reports |
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| Main Authors: | , , , , |
| Format: | Article in Journal/Newspaper |
| Language: | unknown |
| Published: |
Masaryk University Press
2013
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.5817/cpr2013-2-18 https://journals.muni.cz/CPR/article/viewFile/12839/11171 |
| Summary: | A set of nine gram-negative fermenting rods biochemically identified as Escherichia coli was isolated from faeces of seals. These bacteria were characterized by phenotypic classification, 16S rDNA sequence analyses, automated ribotyping, study of whole-cell protein profiles by SDS-PAGE and finally by bacteriocin production. The results of our polyphasic taxonomic study supported the recognition of P4652, P4653 and P4740 isolates as true members of Escherichia albertii species – probably a major enteric human pathogen. To our best knowledge, this is the first evidence showing that E. albertii produces bacteriocin, colicin D. Obtained data unambiguously showed incon-venience of commercial identification systems to distinguish both Escherichia species due to missing data of E. albertii in the commercial databases. The results of Escherichia isolates taxonomy suggest seals as a novel source of human and animal pathogen,E. albertii in the Antarctic region. |
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