Rapid spread of Neisseria gonorrhoeaeciprofloxacin resistance due to a newly introduced resistant strain in Nuuk, Greenland, 2012–2015: a community-based prospective cohort study
Objectives To determine the antimicrobial susceptibility and genotype distribution of Neisseria gonorrhoeae strains isolated from a cohort of patients in Nuuk, Greenland in order to assess the risk of rapid spread in the event of introduction of new strains. Methods Gonococcal isolates (n=102) obtai...
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crjcrbmj:10.1136/bmjopen-2016-011998 2024-06-23T07:53:19+00:00 Rapid spread of Neisseria gonorrhoeaeciprofloxacin resistance due to a newly introduced resistant strain in Nuuk, Greenland, 2012–2015: a community-based prospective cohort study Pedersen, Michael Lynge Poulsen, Peter Berthelsen, Lene Nørgaard, Christina Hoffmann, Steen Jensen, Jørgen Skov 2016 http://dx.doi.org/10.1136/bmjopen-2016-011998 https://syndication.highwire.org/content/doi/10.1136/bmjopen-2016-011998 en eng BMJ BMJ Open volume 6, issue 8, page e011998 ISSN 2044-6055 2044-6055 journal-article 2016 crjcrbmj https://doi.org/10.1136/bmjopen-2016-011998 2024-05-24T13:16:34Z Objectives To determine the antimicrobial susceptibility and genotype distribution of Neisseria gonorrhoeae strains isolated from a cohort of patients in Nuuk, Greenland in order to assess the risk of rapid spread in the event of introduction of new strains. Methods Gonococcal isolates (n=102) obtained from a prospective cohort study of ciprofloxacin resistance were collected between March 2012 and February 2013. Etest minimal inhibitory concentrations (MICs) were determined for ciprofloxacin, azithromycin, ceftriaxone, penicillin, tetracycline, spectinomycin and gentamicin. All isolates were subjected to molecular typing using N. gonorrhoeae multiantigen sequence typing (NG-MAST). After the introduction of a ciprofloxacin-resistant strain in early 2014, an additional 18 isolates were characterised. Results During the study period, all 102 isolates were fully susceptible to ciprofloxacin (≤0.03 mg/L), azithromycin, spectinomycin, gentamicin and ceftriaxone. 10 different NG-MAST types circulated in Nuuk but 7 were found as single isolates, and 3 of the 7 belonged to 1 of the 3 major genogroups (G210, G9816 and G9817) together comprising 96% of the 102 isolates. ST210 accounted for 55% of the 102 strains. The newly introduced ciprofloxacin resistant strain belonged to ST2400 and dominated the population with 59% resistant strains within 6 months after its introduction. All G2400 strains had MICs≥2 mg/L. Conclusions Introduction of a ciprofloxacin-resistant strain into a very homogeneous N. gonorrhoeae population led to an explosive spread of the resistant clone, probably as a result of large sexual networks suggested by the strain homogeneity. Careful surveillance of antimicrobial susceptibility is essential to avoid widespread treatment failure in closed populations. Article in Journal/Newspaper Greenland Nuuk The BMJ Greenland Nuuk ENVELOPE(-52.150,-52.150,68.717,68.717) BMJ Open 6 8 e011998 |
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The BMJ |
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crjcrbmj |
language |
English |
description |
Objectives To determine the antimicrobial susceptibility and genotype distribution of Neisseria gonorrhoeae strains isolated from a cohort of patients in Nuuk, Greenland in order to assess the risk of rapid spread in the event of introduction of new strains. Methods Gonococcal isolates (n=102) obtained from a prospective cohort study of ciprofloxacin resistance were collected between March 2012 and February 2013. Etest minimal inhibitory concentrations (MICs) were determined for ciprofloxacin, azithromycin, ceftriaxone, penicillin, tetracycline, spectinomycin and gentamicin. All isolates were subjected to molecular typing using N. gonorrhoeae multiantigen sequence typing (NG-MAST). After the introduction of a ciprofloxacin-resistant strain in early 2014, an additional 18 isolates were characterised. Results During the study period, all 102 isolates were fully susceptible to ciprofloxacin (≤0.03 mg/L), azithromycin, spectinomycin, gentamicin and ceftriaxone. 10 different NG-MAST types circulated in Nuuk but 7 were found as single isolates, and 3 of the 7 belonged to 1 of the 3 major genogroups (G210, G9816 and G9817) together comprising 96% of the 102 isolates. ST210 accounted for 55% of the 102 strains. The newly introduced ciprofloxacin resistant strain belonged to ST2400 and dominated the population with 59% resistant strains within 6 months after its introduction. All G2400 strains had MICs≥2 mg/L. Conclusions Introduction of a ciprofloxacin-resistant strain into a very homogeneous N. gonorrhoeae population led to an explosive spread of the resistant clone, probably as a result of large sexual networks suggested by the strain homogeneity. Careful surveillance of antimicrobial susceptibility is essential to avoid widespread treatment failure in closed populations. |
format |
Article in Journal/Newspaper |
author |
Pedersen, Michael Lynge Poulsen, Peter Berthelsen, Lene Nørgaard, Christina Hoffmann, Steen Jensen, Jørgen Skov |
spellingShingle |
Pedersen, Michael Lynge Poulsen, Peter Berthelsen, Lene Nørgaard, Christina Hoffmann, Steen Jensen, Jørgen Skov Rapid spread of Neisseria gonorrhoeaeciprofloxacin resistance due to a newly introduced resistant strain in Nuuk, Greenland, 2012–2015: a community-based prospective cohort study |
author_facet |
Pedersen, Michael Lynge Poulsen, Peter Berthelsen, Lene Nørgaard, Christina Hoffmann, Steen Jensen, Jørgen Skov |
author_sort |
Pedersen, Michael Lynge |
title |
Rapid spread of Neisseria gonorrhoeaeciprofloxacin resistance due to a newly introduced resistant strain in Nuuk, Greenland, 2012–2015: a community-based prospective cohort study |
title_short |
Rapid spread of Neisseria gonorrhoeaeciprofloxacin resistance due to a newly introduced resistant strain in Nuuk, Greenland, 2012–2015: a community-based prospective cohort study |
title_full |
Rapid spread of Neisseria gonorrhoeaeciprofloxacin resistance due to a newly introduced resistant strain in Nuuk, Greenland, 2012–2015: a community-based prospective cohort study |
title_fullStr |
Rapid spread of Neisseria gonorrhoeaeciprofloxacin resistance due to a newly introduced resistant strain in Nuuk, Greenland, 2012–2015: a community-based prospective cohort study |
title_full_unstemmed |
Rapid spread of Neisseria gonorrhoeaeciprofloxacin resistance due to a newly introduced resistant strain in Nuuk, Greenland, 2012–2015: a community-based prospective cohort study |
title_sort |
rapid spread of neisseria gonorrhoeaeciprofloxacin resistance due to a newly introduced resistant strain in nuuk, greenland, 2012–2015: a community-based prospective cohort study |
publisher |
BMJ |
publishDate |
2016 |
url |
http://dx.doi.org/10.1136/bmjopen-2016-011998 https://syndication.highwire.org/content/doi/10.1136/bmjopen-2016-011998 |
long_lat |
ENVELOPE(-52.150,-52.150,68.717,68.717) |
geographic |
Greenland Nuuk |
geographic_facet |
Greenland Nuuk |
genre |
Greenland Nuuk |
genre_facet |
Greenland Nuuk |
op_source |
BMJ Open volume 6, issue 8, page e011998 ISSN 2044-6055 2044-6055 |
op_doi |
https://doi.org/10.1136/bmjopen-2016-011998 |
container_title |
BMJ Open |
container_volume |
6 |
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8 |
container_start_page |
e011998 |
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1802644893945823232 |