Relationship of Motility Activation to Lipid Composition, Protein Profile, and Swelling Rate of Burbot Lota lota Spermatozoon Following Change of Temperature and Osmolality

Despite available information on the roles of osmotic pressure, potassium (K + ), and calcium (Ca 2+ ) in activation of Eurasian burbot spermatozoon motility, the changes in milt biochemical composition and mechanisms underlying their activation at temperatures above optimal spawning temperature is...

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Bibliographic Details
Published in:Frontiers in Marine Science
Main Authors: Dadras, Hadiseh, Policar, Tomas, Loginov, Dmitry, Boryshpolets, Sergii, Loginova, Yana, Sampels, Sabine, Sotnikov, Anatolii, Kholodnyy, Vitaliy, Sterba, Jan, Xin, Miaomiao, Dzyuba, Borys
Other Authors: Ministerstvo Zemědělství, Ministerstvo Školství, Mládeže a Tělovýchovy
Format: Article in Journal/Newspaper
Language:unknown
Published: Frontiers Media SA 2021
Subjects:
Ocean Engineering
Water Science and Technology
Aquatic Science
Global and Planetary Change
Oceanography
Burbot
Lota lota
lota
Online Access:http://dx.doi.org/10.3389/fmars.2021.736087
https://www.frontiersin.org/articles/10.3389/fmars.2021.736087/full
Description
Summary:Despite available information on the roles of osmotic pressure, potassium (K + ), and calcium (Ca 2+ ) in activation of Eurasian burbot spermatozoon motility, the changes in milt biochemical composition and mechanisms underlying their activation at temperatures above optimal spawning temperature is still unclear. We explored spermatozoon swelling, lipid composition and proteome in relation to osmolality and temperature of swimming medium. The result revealed that temperature increment from 4 to 30°C increases burbot spermatozoa vulnerability to osmotic pressure, decreasing motility in extremely hypotonic media, and the presence of Ca 2+ decreases swelling of spermatozoa. No spermatozoon swelling was observed in non-ionic isotonic media at all studied temperatures. A role of swelling in activation of spermatozoa motility at 30°C was rejected. No differences were found in protein profile and lipid composition with respect to activation of burbot spermatozoa at 30°C. Burbot spermatozoon activation occurs at 30°C without modification of the spermatozoon membrane. Elucidation of the intrinsic signaling pathway of burbot spermatozoon spontaneous activation requires further study.

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