GATA-3 in Atlantic salmon (Salmo salar): Tissue distribution and its regulation of IL-4/13a promoter
GATA3 is a transcription factor that plays an important role in T cell lineage differentiation and T-helper 2 (Th2) type immune responses. In this study, we developed two rat antibodies against Atlantic salmon GATA-3 (anti-r Ss GATA-3a and anti-r Ss GATA-3b, respectively). The western blotting and i...
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crfrontiers:10.3389/fcimb.2022.1063600 2024-03-31T07:51:41+00:00 GATA-3 in Atlantic salmon (Salmo salar): Tissue distribution and its regulation of IL-4/13a promoter Chi, Heng Meng, Xianghu Dalmo, Roy Ambli National Key Research and Development Program of China National Natural Science Foundation of China 2022 http://dx.doi.org/10.3389/fcimb.2022.1063600 https://www.frontiersin.org/articles/10.3389/fcimb.2022.1063600/full unknown Frontiers Media SA https://creativecommons.org/licenses/by/4.0/ Frontiers in Cellular and Infection Microbiology volume 12 ISSN 2235-2988 Infectious Diseases Microbiology (medical) Immunology Microbiology journal-article 2022 crfrontiers https://doi.org/10.3389/fcimb.2022.1063600 2024-03-05T00:14:59Z GATA3 is a transcription factor that plays an important role in T cell lineage differentiation and T-helper 2 (Th2) type immune responses. In this study, we developed two rat antibodies against Atlantic salmon GATA-3 (anti-r Ss GATA-3a and anti-r Ss GATA-3b, respectively). The western blotting and immunofluorescence results showed that anti-r Ss GATA-3b antibodies recognized endogenous Ss GATA-3 proteins, while the anti-r Ss GATA-3a antibodies did not bind Ss GATA-3. Immunohistochemical analysis revealed that Ss GATA-3 positive cells were detected in all tissues tested, with relatively high number of immune reactive cells in the gills and spleen. Furthermore, the immunohistochemical study revealed that Ss GATA-3 was expressed in pillar cells, epithelial cells, chondrocytes, perichondrium cells, and some undifferentiated basal cells. In addition, we determined 577 bp of the upstream promoter sequence of Ss IL-4/13a and found four motifs that matched Ss GATA-3 binding sites. The promoter regions of Ss IL-4/13a were assessed by transfecting four deletion reporter constructs and Ss GATA-3 overexpression plasmids. The result showed that Ss GATA-3 enhanced the activity of Ss IL-4/13a promoters within the region ranging from -317 to -302 bp upstream of the transcriptional start site. Antibodies against Th2 markers such as GATA-3 are valuable in addressing the diversity of T cell responses in fish. Article in Journal/Newspaper Atlantic salmon Salmo salar Frontiers (Publisher) Gata ENVELOPE(-19.702,-19.702,63.540,63.540) Pillar ENVELOPE(166.217,166.217,-77.583,-77.583) Frontiers in Cellular and Infection Microbiology 12 |
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Open Polar |
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Frontiers (Publisher) |
op_collection_id |
crfrontiers |
language |
unknown |
topic |
Infectious Diseases Microbiology (medical) Immunology Microbiology |
spellingShingle |
Infectious Diseases Microbiology (medical) Immunology Microbiology Chi, Heng Meng, Xianghu Dalmo, Roy Ambli GATA-3 in Atlantic salmon (Salmo salar): Tissue distribution and its regulation of IL-4/13a promoter |
topic_facet |
Infectious Diseases Microbiology (medical) Immunology Microbiology |
description |
GATA3 is a transcription factor that plays an important role in T cell lineage differentiation and T-helper 2 (Th2) type immune responses. In this study, we developed two rat antibodies against Atlantic salmon GATA-3 (anti-r Ss GATA-3a and anti-r Ss GATA-3b, respectively). The western blotting and immunofluorescence results showed that anti-r Ss GATA-3b antibodies recognized endogenous Ss GATA-3 proteins, while the anti-r Ss GATA-3a antibodies did not bind Ss GATA-3. Immunohistochemical analysis revealed that Ss GATA-3 positive cells were detected in all tissues tested, with relatively high number of immune reactive cells in the gills and spleen. Furthermore, the immunohistochemical study revealed that Ss GATA-3 was expressed in pillar cells, epithelial cells, chondrocytes, perichondrium cells, and some undifferentiated basal cells. In addition, we determined 577 bp of the upstream promoter sequence of Ss IL-4/13a and found four motifs that matched Ss GATA-3 binding sites. The promoter regions of Ss IL-4/13a were assessed by transfecting four deletion reporter constructs and Ss GATA-3 overexpression plasmids. The result showed that Ss GATA-3 enhanced the activity of Ss IL-4/13a promoters within the region ranging from -317 to -302 bp upstream of the transcriptional start site. Antibodies against Th2 markers such as GATA-3 are valuable in addressing the diversity of T cell responses in fish. |
author2 |
National Key Research and Development Program of China National Natural Science Foundation of China |
format |
Article in Journal/Newspaper |
author |
Chi, Heng Meng, Xianghu Dalmo, Roy Ambli |
author_facet |
Chi, Heng Meng, Xianghu Dalmo, Roy Ambli |
author_sort |
Chi, Heng |
title |
GATA-3 in Atlantic salmon (Salmo salar): Tissue distribution and its regulation of IL-4/13a promoter |
title_short |
GATA-3 in Atlantic salmon (Salmo salar): Tissue distribution and its regulation of IL-4/13a promoter |
title_full |
GATA-3 in Atlantic salmon (Salmo salar): Tissue distribution and its regulation of IL-4/13a promoter |
title_fullStr |
GATA-3 in Atlantic salmon (Salmo salar): Tissue distribution and its regulation of IL-4/13a promoter |
title_full_unstemmed |
GATA-3 in Atlantic salmon (Salmo salar): Tissue distribution and its regulation of IL-4/13a promoter |
title_sort |
gata-3 in atlantic salmon (salmo salar): tissue distribution and its regulation of il-4/13a promoter |
publisher |
Frontiers Media SA |
publishDate |
2022 |
url |
http://dx.doi.org/10.3389/fcimb.2022.1063600 https://www.frontiersin.org/articles/10.3389/fcimb.2022.1063600/full |
long_lat |
ENVELOPE(-19.702,-19.702,63.540,63.540) ENVELOPE(166.217,166.217,-77.583,-77.583) |
geographic |
Gata Pillar |
geographic_facet |
Gata Pillar |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Frontiers in Cellular and Infection Microbiology volume 12 ISSN 2235-2988 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fcimb.2022.1063600 |
container_title |
Frontiers in Cellular and Infection Microbiology |
container_volume |
12 |
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1795030395416215552 |