GATA-3 in Atlantic salmon (Salmo salar): Tissue distribution and its regulation of IL-4/13a promoter
GATA3 is a transcription factor that plays an important role in T cell lineage differentiation and T-helper 2 (Th2) type immune responses. In this study, we developed two rat antibodies against Atlantic salmon GATA-3 (anti-r Ss GATA-3a and anti-r Ss GATA-3b, respectively). The western blotting and i...
Published in: | Frontiers in Cellular and Infection Microbiology |
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Main Authors: | , , |
Other Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Frontiers Media SA
2022
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Subjects: | |
Online Access: | http://dx.doi.org/10.3389/fcimb.2022.1063600 https://www.frontiersin.org/articles/10.3389/fcimb.2022.1063600/full |
Summary: | GATA3 is a transcription factor that plays an important role in T cell lineage differentiation and T-helper 2 (Th2) type immune responses. In this study, we developed two rat antibodies against Atlantic salmon GATA-3 (anti-r Ss GATA-3a and anti-r Ss GATA-3b, respectively). The western blotting and immunofluorescence results showed that anti-r Ss GATA-3b antibodies recognized endogenous Ss GATA-3 proteins, while the anti-r Ss GATA-3a antibodies did not bind Ss GATA-3. Immunohistochemical analysis revealed that Ss GATA-3 positive cells were detected in all tissues tested, with relatively high number of immune reactive cells in the gills and spleen. Furthermore, the immunohistochemical study revealed that Ss GATA-3 was expressed in pillar cells, epithelial cells, chondrocytes, perichondrium cells, and some undifferentiated basal cells. In addition, we determined 577 bp of the upstream promoter sequence of Ss IL-4/13a and found four motifs that matched Ss GATA-3 binding sites. The promoter regions of Ss IL-4/13a were assessed by transfecting four deletion reporter constructs and Ss GATA-3 overexpression plasmids. The result showed that Ss GATA-3 enhanced the activity of Ss IL-4/13a promoters within the region ranging from -317 to -302 bp upstream of the transcriptional start site. Antibodies against Th2 markers such as GATA-3 are valuable in addressing the diversity of T cell responses in fish. |
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