A novel restriction endonuclease UnbI, a neoschizomer of Sau96I from an unidentified psychrofilic bacterium from Antarctica is inhibited by phosphate ions.

A novel type II restriction endonuclease UnbI was isolated from an unidentified psychrofilic bacterial strain from Antarctica. UnbI recognizes and cleaves the sequence 5'-GGNCC-3', producing 5 nucleotide long sticky ends. In this respect it differs from its neoschizomer Sau96I and all othe...

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Bibliographic Details
Published in:Acta Biochimica Polonica
Main Authors: Kawalec, M, Borsuk, P, Piechula, S, Stepień, P P
Format: Article in Journal/Newspaper
Language:unknown
Published: Frontiers Media SA 1997
Subjects:
General Biochemistry, Genetics and Molecular Biology
Antarc*
Antarctica
Online Access:http://dx.doi.org/10.18388/abp.1997_4390
https://ojs.ptbioch.edu.pl/index.php/abp/article/download/4390/3446
id crfrontiers:10.18388/abp.1997_4390
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spelling crfrontiers:10.18388/abp.1997_4390 2024-02-11T09:57:45+01:00 A novel restriction endonuclease UnbI, a neoschizomer of Sau96I from an unidentified psychrofilic bacterium from Antarctica is inhibited by phosphate ions. Kawalec, M Borsuk, P Piechula, S Stepień, P P 1997 http://dx.doi.org/10.18388/abp.1997_4390 https://ojs.ptbioch.edu.pl/index.php/abp/article/download/4390/3446 unknown Frontiers Media SA Acta Biochimica Polonica volume 44, issue 4, page 849-852 ISSN 1734-154X 0001-527X General Biochemistry, Genetics and Molecular Biology journal-article 1997 crfrontiers https://doi.org/10.18388/abp.1997_4390 2024-01-26T09:59:18Z A novel type II restriction endonuclease UnbI was isolated from an unidentified psychrofilic bacterial strain from Antarctica. UnbI recognizes and cleaves the sequence 5'-GGNCC-3', producing 5 nucleotide long sticky ends. In this respect it differs from its neoschizomer Sau96I and all other restriction enzymes recognizing this sequence. UnbI has a relatively low temperature optimum of 15 degrees C to 20 degrees C and its activity is completely inhibited by inorganic phosphate. Article in Journal/Newspaper Antarc* Antarctica Frontiers (Publisher) Acta Biochimica Polonica 44 4 849 852
institution Open Polar
collection Frontiers (Publisher)
op_collection_id crfrontiers
language unknown
topic General Biochemistry, Genetics and Molecular Biology
spellingShingle General Biochemistry, Genetics and Molecular Biology
Kawalec, M
Borsuk, P
Piechula, S
Stepień, P P
A novel restriction endonuclease UnbI, a neoschizomer of Sau96I from an unidentified psychrofilic bacterium from Antarctica is inhibited by phosphate ions.
topic_facet General Biochemistry, Genetics and Molecular Biology
description A novel type II restriction endonuclease UnbI was isolated from an unidentified psychrofilic bacterial strain from Antarctica. UnbI recognizes and cleaves the sequence 5'-GGNCC-3', producing 5 nucleotide long sticky ends. In this respect it differs from its neoschizomer Sau96I and all other restriction enzymes recognizing this sequence. UnbI has a relatively low temperature optimum of 15 degrees C to 20 degrees C and its activity is completely inhibited by inorganic phosphate.
format Article in Journal/Newspaper
author Kawalec, M
Borsuk, P
Piechula, S
Stepień, P P
author_facet Kawalec, M
Borsuk, P
Piechula, S
Stepień, P P
author_sort Kawalec, M
title A novel restriction endonuclease UnbI, a neoschizomer of Sau96I from an unidentified psychrofilic bacterium from Antarctica is inhibited by phosphate ions.
title_short A novel restriction endonuclease UnbI, a neoschizomer of Sau96I from an unidentified psychrofilic bacterium from Antarctica is inhibited by phosphate ions.
title_full A novel restriction endonuclease UnbI, a neoschizomer of Sau96I from an unidentified psychrofilic bacterium from Antarctica is inhibited by phosphate ions.
title_fullStr A novel restriction endonuclease UnbI, a neoschizomer of Sau96I from an unidentified psychrofilic bacterium from Antarctica is inhibited by phosphate ions.
title_full_unstemmed A novel restriction endonuclease UnbI, a neoschizomer of Sau96I from an unidentified psychrofilic bacterium from Antarctica is inhibited by phosphate ions.
title_sort novel restriction endonuclease unbi, a neoschizomer of sau96i from an unidentified psychrofilic bacterium from antarctica is inhibited by phosphate ions.
publisher Frontiers Media SA
publishDate 1997
url http://dx.doi.org/10.18388/abp.1997_4390
https://ojs.ptbioch.edu.pl/index.php/abp/article/download/4390/3446
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_source Acta Biochimica Polonica
volume 44, issue 4, page 849-852
ISSN 1734-154X 0001-527X
op_doi https://doi.org/10.18388/abp.1997_4390
container_title Acta Biochimica Polonica
container_volume 44
container_issue 4
container_start_page 849
op_container_end_page 852
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