A novel restriction endonuclease UnbI, a neoschizomer of Sau96I from an unidentified psychrofilic bacterium from Antarctica is inhibited by phosphate ions.
A novel type II restriction endonuclease UnbI was isolated from an unidentified psychrofilic bacterial strain from Antarctica. UnbI recognizes and cleaves the sequence 5'-GGNCC-3', producing 5 nucleotide long sticky ends. In this respect it differs from its neoschizomer Sau96I and all othe...
Published in: | Acta Biochimica Polonica |
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Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Frontiers Media SA
1997
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Subjects: | |
Online Access: | http://dx.doi.org/10.18388/abp.1997_4390 https://ojs.ptbioch.edu.pl/index.php/abp/article/download/4390/3446 |
Summary: | A novel type II restriction endonuclease UnbI was isolated from an unidentified psychrofilic bacterial strain from Antarctica. UnbI recognizes and cleaves the sequence 5'-GGNCC-3', producing 5 nucleotide long sticky ends. In this respect it differs from its neoschizomer Sau96I and all other restriction enzymes recognizing this sequence. UnbI has a relatively low temperature optimum of 15 degrees C to 20 degrees C and its activity is completely inhibited by inorganic phosphate. |
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