A novel restriction endonuclease UnbI, a neoschizomer of Sau96I from an unidentified psychrofilic bacterium from Antarctica is inhibited by phosphate ions.

A novel type II restriction endonuclease UnbI was isolated from an unidentified psychrofilic bacterial strain from Antarctica. UnbI recognizes and cleaves the sequence 5'-GGNCC-3', producing 5 nucleotide long sticky ends. In this respect it differs from its neoschizomer Sau96I and all othe...

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Bibliographic Details
Published in:Acta Biochimica Polonica
Main Authors: Kawalec, M, Borsuk, P, Piechula, S, Stepień, P P
Format: Article in Journal/Newspaper
Language:unknown
Published: Frontiers Media SA 1997
Subjects:
General Biochemistry, Genetics and Molecular Biology
Antarc*
Antarctica
Online Access:http://dx.doi.org/10.18388/abp.1997_4390
https://ojs.ptbioch.edu.pl/index.php/abp/article/download/4390/3446
Description
Summary:A novel type II restriction endonuclease UnbI was isolated from an unidentified psychrofilic bacterial strain from Antarctica. UnbI recognizes and cleaves the sequence 5'-GGNCC-3', producing 5 nucleotide long sticky ends. In this respect it differs from its neoschizomer Sau96I and all other restriction enzymes recognizing this sequence. UnbI has a relatively low temperature optimum of 15 degrees C to 20 degrees C and its activity is completely inhibited by inorganic phosphate.

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