The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

Heterochromatic domains are enriched with repressive histone marks, including histone H3 lysine 9 methylation, written by lysine methyltransferases (KMTs). The pre-replication complex protein, origin recognition complex-associated (ORCA/LRWD1), preferentially localizes to heterochromatic regions in...

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Bibliographic Details
Published in:eLife
Main Authors: Giri, Sumanprava, Aggarwal, Vasudha, Pontis, Julien, Shen, Zhen, Chakraborty, Arindam, Khan, Abid, Mizzen, Craig, Prasanth, Kannanganattu V, Ait-Si-Ali, Slimane, Ha, Taekjip, Prasanth, Supriya G
Other Authors: National Institutes of Health (NIH), National Science Foundation (NSF), Howard Hughes Medical Institute (HHMI), Agence Nationale de la Recherche, AFM-Téléthon (French Muscular Dystrophy Association), Fondation ARC pour la Recherche sur le Cancer, Institut national du cancer, Ministère de l'Education Nationale, de l'Enseignement Superieur et de la Recherche, American Cancer Society
Format: Article in Journal/Newspaper
Language:English
Published: eLife Sciences Publications, Ltd 2015
Subjects:
Orca
Online Access:http://dx.doi.org/10.7554/elife.06496
http://elifesciences.org/lookup/doi/10.7554/eLife.06496
https://cdn.elifesciences.org/articles/06496/elife-06496-v2.pdf
https://cdn.elifesciences.org/articles/06496/elife-06496-v2.xml
https://elifesciences.org/articles/06496
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Summary:Heterochromatic domains are enriched with repressive histone marks, including histone H3 lysine 9 methylation, written by lysine methyltransferases (KMTs). The pre-replication complex protein, origin recognition complex-associated (ORCA/LRWD1), preferentially localizes to heterochromatic regions in post-replicated cells. Its role in heterochromatin organization remained elusive. ORCA recognizes methylated H3K9 marks and interacts with repressive KMTs, including G9a/GLP and Suv39H1 in a chromatin context-dependent manner. Single-molecule pull-down assays demonstrate that ORCA-ORC (Origin Recognition Complex) and multiple H3K9 KMTs exist in a single complex and that ORCA stabilizes H3K9 KMT complex. Cells lacking ORCA show alterations in chromatin architecture, with significantly reduced H3K9 di- and tri-methylation at specific chromatin sites. Changes in heterochromatin structure due to loss of ORCA affect replication timing, preferentially at the late-replicating regions. We demonstrate that ORCA acts as a scaffold for the establishment of H3K9 KMT complex and its association and activity at specific chromatin sites is crucial for the organization of heterochromatin structure.

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