Trehalose improves the movement ability of Aβ arc Drosophila by restoring the damaged mitochondria
Abstract Background The deposition of Aβ 42 has been regarded as one of the important pathological features of Alzheimer’s disease (AD). However, drug development for Aβ 42 toxicity has been progressed slowly. Objective Our aim was to introduce the effect and related mechanism of trehalose on an Aβ...
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crdegruyter:10.1515/tnsci-2022-0338 2024-05-12T08:00:11+00:00 Trehalose improves the movement ability of Aβ arc Drosophila by restoring the damaged mitochondria Li, Liangxian Huang, Zhiheng Wu, Mingli Li, Xia Xiao, Bo Yao, Dong Mo, Biwen 2024 http://dx.doi.org/10.1515/tnsci-2022-0338 https://www.degruyter.com/document/doi/10.1515/tnsci-2022-0338/xml https://www.degruyter.com/document/doi/10.1515/tnsci-2022-0338/pdf en eng Walter de Gruyter GmbH http://creativecommons.org/licenses/by/4.0 Translational Neuroscience volume 15, issue 1 ISSN 2081-6936 General Neuroscience journal-article 2024 crdegruyter https://doi.org/10.1515/tnsci-2022-0338 2024-04-18T06:55:34Z Abstract Background The deposition of Aβ 42 has been regarded as one of the important pathological features of Alzheimer’s disease (AD). However, drug development for Aβ 42 toxicity has been progressed slowly. Objective Our aim was to introduce the effect and related mechanism of trehalose on an Aβ arc (arctic mutant Aβ 42 ) Drosophila AD model. Methods The human Aβ arc was expressed in Drosophila to construct the AD model. Trehalose was added to the culture vial. The movement ability was determined by detecting climbing ability and flight ability. Enzyme-linked immunosorbent assay was used to detect the levels of Aβ arc , ATP, and lactate. Electron microscopy assay, mitochondrial membrane potential assay, and mitochondrial respiration assay were used to assess the mitochondrial structure and function. Results Trehalose strongly improved the movement ability of Aβ arc Drosophila in a concentration gradient-dependent manner. Furthermore, trehalose increased the content of ATP and decreased the content of Aβ arc and lactate both in the brain and thorax of Aβ arc Drosophila . More importantly, the mitochondrial structure and function were greatly improved by trehalose treatment in Aβ arc Drosophila . Conclusion Trehalose improves movement ability at least partly by reducing the Aβ arc level and restoring the mitochondrial structure and function in Aβ arc Drosophila . Article in Journal/Newspaper Arctic De Gruyter Arctic Translational Neuroscience 15 1 |
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crdegruyter |
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English |
topic |
General Neuroscience |
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General Neuroscience Li, Liangxian Huang, Zhiheng Wu, Mingli Li, Xia Xiao, Bo Yao, Dong Mo, Biwen Trehalose improves the movement ability of Aβ arc Drosophila by restoring the damaged mitochondria |
topic_facet |
General Neuroscience |
description |
Abstract Background The deposition of Aβ 42 has been regarded as one of the important pathological features of Alzheimer’s disease (AD). However, drug development for Aβ 42 toxicity has been progressed slowly. Objective Our aim was to introduce the effect and related mechanism of trehalose on an Aβ arc (arctic mutant Aβ 42 ) Drosophila AD model. Methods The human Aβ arc was expressed in Drosophila to construct the AD model. Trehalose was added to the culture vial. The movement ability was determined by detecting climbing ability and flight ability. Enzyme-linked immunosorbent assay was used to detect the levels of Aβ arc , ATP, and lactate. Electron microscopy assay, mitochondrial membrane potential assay, and mitochondrial respiration assay were used to assess the mitochondrial structure and function. Results Trehalose strongly improved the movement ability of Aβ arc Drosophila in a concentration gradient-dependent manner. Furthermore, trehalose increased the content of ATP and decreased the content of Aβ arc and lactate both in the brain and thorax of Aβ arc Drosophila . More importantly, the mitochondrial structure and function were greatly improved by trehalose treatment in Aβ arc Drosophila . Conclusion Trehalose improves movement ability at least partly by reducing the Aβ arc level and restoring the mitochondrial structure and function in Aβ arc Drosophila . |
format |
Article in Journal/Newspaper |
author |
Li, Liangxian Huang, Zhiheng Wu, Mingli Li, Xia Xiao, Bo Yao, Dong Mo, Biwen |
author_facet |
Li, Liangxian Huang, Zhiheng Wu, Mingli Li, Xia Xiao, Bo Yao, Dong Mo, Biwen |
author_sort |
Li, Liangxian |
title |
Trehalose improves the movement ability of Aβ arc Drosophila by restoring the damaged mitochondria |
title_short |
Trehalose improves the movement ability of Aβ arc Drosophila by restoring the damaged mitochondria |
title_full |
Trehalose improves the movement ability of Aβ arc Drosophila by restoring the damaged mitochondria |
title_fullStr |
Trehalose improves the movement ability of Aβ arc Drosophila by restoring the damaged mitochondria |
title_full_unstemmed |
Trehalose improves the movement ability of Aβ arc Drosophila by restoring the damaged mitochondria |
title_sort |
trehalose improves the movement ability of aβ arc drosophila by restoring the damaged mitochondria |
publisher |
Walter de Gruyter GmbH |
publishDate |
2024 |
url |
http://dx.doi.org/10.1515/tnsci-2022-0338 https://www.degruyter.com/document/doi/10.1515/tnsci-2022-0338/xml https://www.degruyter.com/document/doi/10.1515/tnsci-2022-0338/pdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Translational Neuroscience volume 15, issue 1 ISSN 2081-6936 |
op_rights |
http://creativecommons.org/licenses/by/4.0 |
op_doi |
https://doi.org/10.1515/tnsci-2022-0338 |
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Translational Neuroscience |
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15 |
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