Trehalose improves the movement ability of Aβ arc Drosophila by restoring the damaged mitochondria

• Published in: Translational Neuroscience
• Main Authors: Li, Liangxian, Huang, Zhiheng, Wu, Mingli, Li, Xia, Xiao, Bo, Yao, Dong, Mo, Biwen
• Format: Article in Journal/Newspaper
• Language: English
• Published: Walter de Gruyter GmbH 2024
• Subjects: General Neuroscience; Arctic
• Online Access: http://dx.doi.org/10.1515/tnsci-2022-0338; https://www.degruyter.com/document/doi/10.1515/tnsci-2022-0338/xml; https://www.degruyter.com/document/doi/10.1515/tnsci-2022-0338/pdf

Abstract Background The deposition of Aβ 42 has been regarded as one of the important pathological features of Alzheimer’s disease (AD). However, drug development for Aβ 42 toxicity has been progressed slowly. Objective Our aim was to introduce the effect and related mechanism of trehalose on an Aβ arc (arctic mutant Aβ 42 ) Drosophila AD model. Methods The human Aβ arc was expressed in Drosophila to construct the AD model. Trehalose was added to the culture vial. The movement ability was determined by detecting climbing ability and flight ability. Enzyme-linked immunosorbent assay was used to detect the levels of Aβ arc , ATP, and lactate. Electron microscopy assay, mitochondrial membrane potential assay, and mitochondrial respiration assay were used to assess the mitochondrial structure and function. Results Trehalose strongly improved the movement ability of Aβ arc Drosophila in a concentration gradient-dependent manner. Furthermore, trehalose increased the content of ATP and decreased the content of Aβ arc and lactate both in the brain and thorax of Aβ arc Drosophila . More importantly, the mitochondrial structure and function were greatly improved by trehalose treatment in Aβ arc Drosophila . Conclusion Trehalose improves movement ability at least partly by reducing the Aβ arc level and restoring the mitochondrial structure and function in Aβ arc Drosophila .