Purification and characterization of a novel α-glucosidase from an Antarctic yeast Dioszegia fristingensis isolate
Abstract Starch hydrolyzing enzymes, amylases, are important commercial enzymes used in several productive areas. A current tendency is to find amylases with high catalytic activity at 20-40°C, to generate products that work well at low temperatures, such as detergents, and for energy saving resourc...
| Published in: | Amylase |
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| Main Authors: | , , , |
| Format: | Article in Journal/Newspaper |
| Language: | unknown |
| Published: |
Walter de Gruyter GmbH
2017
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1515/amylase-2017-0005 http://www.degruyter.com/view/j/amylase.2017.1.issue-1/amylase-2017-0005/amylase-2017-0005.xml https://www.degruyter.com/document/doi/10.1515/amylase-2017-0005/pdf |
| Summary: | Abstract Starch hydrolyzing enzymes, amylases, are important commercial enzymes used in several productive areas. A current tendency is to find amylases with high catalytic activity at 20-40°C, to generate products that work well at low temperatures, such as detergents, and for energy saving resources in industrial processes. In this work, an α-glucosidase secreted by the cold-adapted yeast Dioszegia fristingensis was purified and biochemically characterized. The effect of physicochemical parameters on the enzyme activity was evaluated. According to our results, the amylolytic enzyme secreted by D. fristingensis is a monomeric α-glucosidase of about 30 kDa that displayed the highest activity at 37-40°C and at pH 5.5-6.5,in the presence of 10 mM CaCl |
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