Heterogeneity of antifreeze polypeptides from the Newfoundland winter flounder, Pseudopleuronectes americanus

The heterogeneity of Newfoundland winter flounder antifreeze polypeptides was analyzed by reverse phase high performance liquid chromatography and by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Seven antifreeze polypeptide components could be readily demonstrated. Five of the componen...

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Bibliographic Details
Published in:Canadian Journal of Zoology
Main Authors: Fourney, Ron M., Joshi, Shashikant B., Kao, Ming H., Hew, Choy L.
Format: Article in Journal/Newspaper
Language:English
Published: Canadian Science Publishing 1984
Subjects:
Animal Science and Zoology
Ecology, Evolution, Behavior and Systematics
Newfoundland
Online Access:http://dx.doi.org/10.1139/z84-006
http://www.nrcresearchpress.com/doi/pdf/10.1139/z84-006
Description
Summary:The heterogeneity of Newfoundland winter flounder antifreeze polypeptides was analyzed by reverse phase high performance liquid chromatography and by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Seven antifreeze polypeptide components could be readily demonstrated. Five of the components were similar in molecular weight (3300) and amino acid composition. Two of the antifreeze polypeptide components were larger (4500) and contained valine. The two major components (components 6 and 8) were identical to those reported earlier from our laboratories.

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