Determining lactose content of harp seal milk

Lactose levels in harp seal (Phoca groenlandica) milk were estimated using three quantitative techniques (phenol–sulphuric acid, anthrone reagent, and picric acid). Based on its low coefficient of variation, accuracy, and simplicity, the anthrone technique was the preferred method. Lactose content o...

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Bibliographic Details
Published in:Canadian Journal of Zoology
Main Authors: Stewart, R. E. A., Webb, B. E., Lavigne, D. M., Fletcher, F.
Format: Article in Journal/Newspaper
Language:English
Published: Canadian Science Publishing 1983
Subjects:
Online Access:http://dx.doi.org/10.1139/z83-143
http://www.nrcresearchpress.com/doi/pdf/10.1139/z83-143
Description
Summary:Lactose levels in harp seal (Phoca groenlandica) milk were estimated using three quantitative techniques (phenol–sulphuric acid, anthrone reagent, and picric acid). Based on its low coefficient of variation, accuracy, and simplicity, the anthrone technique was the preferred method. Lactose content of harp seal milk remained constant throughout lactation at 0.89 ± 0.14 g per 100 g whole milk ([Formula: see text], n = 22). Fat, water, and protein content of our samples were not significantly different from those determined in a previous study. The absence of between-year differences in these constituents further suggests that carbohydrate content also did not change significantly from year to year.