Reductive dechlorination of weathered Aroclor 1260 during anaerobic biotreatment of Arctic soils

• Published in: Canadian Journal of Microbiology
• Main Authors: Kuipers, Bianca, Cullen, William R, Mohn, William W
• Format: Article in Journal/Newspaper
• Language: English
• Published: Canadian Science Publishing 2003
• Subjects: Nunavut
• Online Access: http://dx.doi.org/10.1139/w03-005; http://www.nrcresearchpress.com/doi/pdf/10.1139/w03-005

We investigated the microbial reductive dechlorination of both weathered (aged) and nonweathered (freshly added) Aroclor 1260 in aerobic soil from Resolution Island, Nunavut, Canada. Initial polychlorinated biphenyl (PCB) concentrations were 106 and 100 ppm, respectively. The aerobic soil samples were inoculated with anaerobic sediment, incubated at 30°C until methanogenic, inoculated with a dechlorinating enrichment culture, and incubated a further 8 weeks. The average number of chlorine substituents per biphenyl molecule was biologically reduced from 6.6 to 5.1 and from 6.2 to 4.5 for weathered and nonweathered Aroclor 1260, respectively. Removal of hexa- and heptachlorobiphenyls (CBs), the major homolog groups present, was significantly greater for nonweathered than for weathered Aroclor 1260. Formation of dechlorination products, primarily 2,2',4,4'- and 2,2',4,6'-tetraCBs, was also significantly greater for nonweathered than for weathered Aroclor 1260. We additionally compared the dechlorination at 21°C of weathered Aroclor 1260 in soils from Resolution Island and Saglek, Labrador, Canada. The average number of chlorine substituents per biphenyl molecule was biologically reduced from 6.7 to 5.1 and from 6.5 to 4.6, respectively. This study demonstrated the potential for bioremediation of aerobic soil contaminated with Aroclor 1260 and showed that weathering may limit such treatment to an extent variable among different soils.Key words: biodegradation, PCB, polychlorinated biphenyl, bioremediation.