Co-culture of Aeromonas salmonicida and host cells in intraperitoneal implants is associated with enhanced bacterial survival
An experimental procedure that we named "in vivo co-culture technology" allowed us to study the interactions between Aeromonas salmonicida and host cells, inside semipermeable chambers implanted in the peritoneal cavity of Atlantic salmon. Intraperitoneal implants containing bacteria and h...
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Online Access: | http://dx.doi.org/10.1139/w00-033 http://www.nrcresearchpress.com/doi/pdf/10.1139/w00-033 |
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crcansciencepubl:10.1139/w00-033 2023-12-17T10:27:24+01:00 Co-culture of Aeromonas salmonicida and host cells in intraperitoneal implants is associated with enhanced bacterial survival Garduño, Rafael A Lizama, Angela L Moore, Anne R Garduño, Elizabeth Olivier, Gilles Kay, William W 2000 http://dx.doi.org/10.1139/w00-033 http://www.nrcresearchpress.com/doi/pdf/10.1139/w00-033 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 46, issue 7, page 674-678 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 2000 crcansciencepubl https://doi.org/10.1139/w00-033 2023-11-19T13:39:09Z An experimental procedure that we named "in vivo co-culture technology" allowed us to study the interactions between Aeromonas salmonicida and host cells, inside semipermeable chambers implanted in the peritoneal cavity of Atlantic salmon. Intraperitoneal implants containing bacteria and host cells, or bacteria and lysed cells, consistently yielded higher numbers of viable bacteria than implants containing bacteria only. Electron microscopy confirmed that 30 min after chamber inoculation, numerous bacteria were already internalized by exudate cells, and that at 3 h, destruction of these cells was evident. Thus, the rapid invasion and (or) the A. salmonicida-mediated lysis of host cells may constitute a survival strategy in vivo. The co-culture of bacteria with exudate peritoneal cells may be applicable to the in vivo study of other pathogens.Key words: Aeromonas salmonicida, in vivo, intraperitoneal implants, exudate cells, furunculosis. Article in Journal/Newspaper Atlantic salmon Canadian Science Publishing (via Crossref) Canadian Journal of Microbiology 46 7 674 678 |
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Canadian Science Publishing (via Crossref) |
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crcansciencepubl |
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English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
spellingShingle |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology Garduño, Rafael A Lizama, Angela L Moore, Anne R Garduño, Elizabeth Olivier, Gilles Kay, William W Co-culture of Aeromonas salmonicida and host cells in intraperitoneal implants is associated with enhanced bacterial survival |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
An experimental procedure that we named "in vivo co-culture technology" allowed us to study the interactions between Aeromonas salmonicida and host cells, inside semipermeable chambers implanted in the peritoneal cavity of Atlantic salmon. Intraperitoneal implants containing bacteria and host cells, or bacteria and lysed cells, consistently yielded higher numbers of viable bacteria than implants containing bacteria only. Electron microscopy confirmed that 30 min after chamber inoculation, numerous bacteria were already internalized by exudate cells, and that at 3 h, destruction of these cells was evident. Thus, the rapid invasion and (or) the A. salmonicida-mediated lysis of host cells may constitute a survival strategy in vivo. The co-culture of bacteria with exudate peritoneal cells may be applicable to the in vivo study of other pathogens.Key words: Aeromonas salmonicida, in vivo, intraperitoneal implants, exudate cells, furunculosis. |
format |
Article in Journal/Newspaper |
author |
Garduño, Rafael A Lizama, Angela L Moore, Anne R Garduño, Elizabeth Olivier, Gilles Kay, William W |
author_facet |
Garduño, Rafael A Lizama, Angela L Moore, Anne R Garduño, Elizabeth Olivier, Gilles Kay, William W |
author_sort |
Garduño, Rafael A |
title |
Co-culture of Aeromonas salmonicida and host cells in intraperitoneal implants is associated with enhanced bacterial survival |
title_short |
Co-culture of Aeromonas salmonicida and host cells in intraperitoneal implants is associated with enhanced bacterial survival |
title_full |
Co-culture of Aeromonas salmonicida and host cells in intraperitoneal implants is associated with enhanced bacterial survival |
title_fullStr |
Co-culture of Aeromonas salmonicida and host cells in intraperitoneal implants is associated with enhanced bacterial survival |
title_full_unstemmed |
Co-culture of Aeromonas salmonicida and host cells in intraperitoneal implants is associated with enhanced bacterial survival |
title_sort |
co-culture of aeromonas salmonicida and host cells in intraperitoneal implants is associated with enhanced bacterial survival |
publisher |
Canadian Science Publishing |
publishDate |
2000 |
url |
http://dx.doi.org/10.1139/w00-033 http://www.nrcresearchpress.com/doi/pdf/10.1139/w00-033 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Canadian Journal of Microbiology volume 46, issue 7, page 674-678 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/w00-033 |
container_title |
Canadian Journal of Microbiology |
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46 |
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7 |
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674 |
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678 |
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