Discriminating between dispersion and lyoprotection effects in biocatalysis in organic media

The increment of activity and solubility in 1,4-dioxane of lipase B from Candida antarctica, lipase from Pseudomonas cepacia, and subtilisin, were investigated as a function of the methoxypoly(ethylene glycol)–protein (PEG–protein) ratio employed during lyophilization. Both activity and solubility m...

Full description

Bibliographic Details
Published in:Canadian Journal of Chemistry
Main Authors: Secundo, Francesco, Carrea, Giacomo, Veronese, Francesco Maria
Format: Article in Journal/Newspaper
Language:English
Published: Canadian Science Publishing 2002
Subjects:
Organic Chemistry
General Chemistry
Catalysis
Antarc*
Antarctica
Online Access:http://dx.doi.org/10.1139/v02-026
http://www.nrcresearchpress.com/doi/pdf/10.1139/v02-026
Description
Summary:The increment of activity and solubility in 1,4-dioxane of lipase B from Candida antarctica, lipase from Pseudomonas cepacia, and subtilisin, were investigated as a function of the methoxypoly(ethylene glycol)–protein (PEG–protein) ratio employed during lyophilization. Both activity and solubility markedly increased as the PEG–protein ratio was increased. The increment of activity at low PEG–protein ratios, however, was much higher than that of solubility. These data suggest that the PEG-induced activation effect is due mainly to a lyoprotection effect rather than to relaxation of diffusional limitations.Key words: hydrolases, activity, dispersion, lyoprotection, circular dichroism.

Similar Items