An enation system for the isolation of Antarctic yeasts inhibited by conventional media

Yeasts peculiar to the Dry Valleys of Antarctica can be recovered by allowing them to become enate (grow out) from soil particles. The enation system was successful in liquid medium E-1 shaken at 160 rpm in a 10 °C water bath. Samples were spread on a richer medium, 2 MC. This system also allowed th...

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Bibliographic Details
Published in:Canadian Journal of Microbiology
Main Author: Vishniac, H. S.
Format: Article in Journal/Newspaper
Language:English
Published: Canadian Science Publishing 1983
Subjects:
Genetics
Molecular Biology
Applied Microbiology and Biotechnology
General Medicine
Immunology
Microbiology
Antarctic
Antarc*
Antarctica
Online Access:http://dx.doi.org/10.1139/m83-014
http://www.nrcresearchpress.com/doi/pdf/10.1139/m83-014
Description
Summary:Yeasts peculiar to the Dry Valleys of Antarctica can be recovered by allowing them to become enate (grow out) from soil particles. The enation system was successful in liquid medium E-1 shaken at 160 rpm in a 10 °C water bath. Samples were spread on a richer medium, 2 MC. This system also allowed the isolation of typical soil yeasts from Colorado soils and of cyanobacteria, heterotrophic bacteria, filamentous fungi, and chlorophyta. Marked inhibitory effects of conventional yeast media account for the failure of previous investigators to isolate these Antarctic yeasts. Their abundance remains unknown, since E-1 and 2 MC also have some inhibitory effects.

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