Isolation, characterization, and chromosomal location of the tRNA Met genes in Atlantic salmon ( Salmo salar ) and brown trout ( Salmo trutta )

This work describes the isolation, characterization, and physical location of the methionine tRNA in the genome of Atlantic salmon (Salmo salar L.) and brown trout (Salmo trutta L.). An Atlantic salmon genomic library was screened using a tRNA Met probe from Xenopus laevis. Two cosmid clones contain...

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Bibliographic Details
Published in:Genome
Main Authors: Perez, J, Moran, P, Garcia-Vazquez, E
Format: Article in Journal/Newspaper
Language:English
Published: Canadian Science Publishing 2000
Subjects:
Genetics
Molecular Biology
General Medicine
Biotechnology
Atlantic salmon
Salmo salar
Online Access:http://dx.doi.org/10.1139/g99-084
http://www.nrcresearchpress.com/doi/pdf/10.1139/g99-084
Description
Summary:This work describes the isolation, characterization, and physical location of the methionine tRNA in the genome of Atlantic salmon (Salmo salar L.) and brown trout (Salmo trutta L.). An Atlantic salmon genomic library was screened using a tRNA Met probe from Xenopus laevis. Two cosmid clones containing the Atlantic salmon tRNA Met gene were isolated, subcloned and sequenced. The tRNA Met was mapped to metaphase chromosomes by fluorescence in situ hybridization (FISH). Chromosomal data indicated that the tDNA of methionine is tandemly repeated in a single locus in both species. Analysis of genomic DNA by Southern hybridization confirmed the tandem organization of this gene. Key words: cosmids, cloning, in situ hybridization, tRNA Met .

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