Chromosomes and DNA of Anguilla anguilla : a study with restriction endonucleases

We have studied fixed chromosomes and purified DNA of Anguilla anguilla L. after digestion with HaeIII, AluI, MboI, and DdeI restriction endonucleases. Our data demonstrated (i) confirmation of the heteromorphic nature of NORs, (ii) absence of detectable sex chromosomes, and (iii) presence of discre...

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Bibliographic Details
Published in:Genome
Main Authors: Cau, A., Coluccia, E., Deiana, A. M., Pichiri, G., Rossino, R., Salvadori, S., Mezzanotte, R.
Format: Article in Journal/Newspaper
Language:English
Published: Canadian Science Publishing 1992
Subjects:
Genetics
Molecular Biology
General Medicine
Biotechnology
Anguilla anguilla
Online Access:http://dx.doi.org/10.1139/g92-127
http://www.nrcresearchpress.com/doi/pdf/10.1139/g92-127
Description
Summary:We have studied fixed chromosomes and purified DNA of Anguilla anguilla L. after digestion with HaeIII, AluI, MboI, and DdeI restriction endonucleases. Our data demonstrated (i) confirmation of the heteromorphic nature of NORs, (ii) absence of detectable sex chromosomes, and (iii) presence of discrete intercalary domains in this species. Our data also permitted us to hypothesize the existence of highly repetitive DNAs, localized in specific heterochromatic regions of A. anguilla chromosomes.Key words: fish, chromosomes, DNA, restriction enzymes.

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